中药四性的生物热动力学研究(Ⅱ) 参叶和参花药性的微量量热学比较  

Biothermodynamic study on four therapeutic features of TCM Ⅱ Comparison of therapeutic features of Folium ginseng and Flos ginseng by microcalorimetry

作　　者：余惠旻[1] 肖小河[2] 刘塔斯[1] 赵艳玲[2] 袁海龙[2] 谭安民[3] 高晓山[4] 

机构地区：[1]湖南中医学院药学院,湖南长沙410007 [2]解放军302医院药学部,北京100039 [3]北京医科大学天然药物及仿生药物国家重点实验室,北京100083 [4]中国中医研究院中药研究所,北京100700

出　　处：《中草药》2001年第10期910-913,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目 ( 39970 911)

摘　　要：目的　从生物物理化学的角度 ,阐明中药四性 (寒、热、温、凉 )的客观真实性。方法　利用微量量热法 ,测定了大肠杆菌在不同条件下的生长热谱曲线 ,得到了相应的生物热动力学参数如生长速率常数 (K)、抑制率 (I)、半抑制率 (IC50 )、热焓 (ΔH )。根据生物热动力学通用数值 ,结合本草文献报道 ,综合分析了参叶和参花药性的差异所在。结果　参叶和参花抑制细菌生长的热谱曲线 ,其形状基本相同 ,随着药物浓度的加大 ,代谢过程的停滞期延长 ,生长代谢峰后移 ;其热力学参数热焓 (Δ H)存在较稳定的差异——参叶降低了细菌生长代谢过程中热量的释放 ,而参花增加了细菌生长代谢过程中热量的释放。结论　微量量热法可作为刻划中药药性的一个新的有效手段 。Object To demonstrate the naked truth of four therapeutic features of TCM (cold, hot, warm and cool) from biophysical and biochemical point of view. Methods Growth thermogenesis curves of Escherichia coli were determined under different conditions by microcalorimetry to give biothermodynamic parameters, such as multiplication constant (k), median inhibitory concentration (IC 50 ), and enthalpy (ΔH). Differences between folia ginseng and flos ginseng were comprehensively compared with reference to classical Chinese literatures on material medica. Results The bacteriostatic curve of both drugs were basically similar. With increasing concentration of decoctions of both drugs, the lag phase was delayed and the peaks shifted to the right. While the difference of ΔH was stable, which decreases with the increasing concentration of Folium ginseng, but increases with increasing concentration of Flos ginseng. Conclusion Microcalorimetry is not only a new effective method to describe the therapeutic features of TCM, but also could be used as a tool for the screening of active TCM.

关 键 词：中药四性 微量量热法 参叶 参花 热焓 

分 类 号：R285.1[医药卫生—中药学]