消斑肽加速氧化低密度脂蛋白诱导的血管平滑肌细胞凋亡  

XBP-01 accelerated apoptosis induced by oxidized low density lipoprotein in vascular smooth muscle cells

作　　者：袁中华[1] 杨永宗[1] 杨小毅[1] 谭健苗[1] 万载阳[1] 

机构地区：[1]衡阳医学院心血管病研究所,湖南衡阳421001

出　　处：《中国病理生理杂志》2001年第10期935-937,T001,共4页Chinese Journal of Pathophysiology

基　　金：湖南省自然科学基金资助项目 (No.97JJY2 0 2 5 )

摘　　要：目的 :我们已经发现消斑肽能逆转平滑肌源性的泡沫细胞 ,阻抑C5 7BL/6J小鼠动脉粥样硬化斑块的形成 ,对已形成的斑块有消退作用。本文主要探讨消斑肽的作用机制。方法 :体外培养的猪主动脉平滑肌细胞 ,先与 15mg/L的氧化低密度脂蛋白孵育 72h ,再与 0 1mg/L消斑肽孵育 2 4h ,应用荧光染色技术、激光共聚焦显微技术和流式细胞术 ,观察消斑肽对平滑肌细胞的作用。结果 :氧化低密度脂蛋白能诱导血管平滑肌细胞发生凋亡 ;消斑肽能加速氧化低密度脂蛋白诱导的细胞凋亡。结论 :综合以前的实验 ,消斑肽可能是通过加速斑块中细胞的凋亡发挥抗动脉粥样硬化效果。AIM: Previous studies performed with XBP-01 in vitro indicated that XBP-01 could inhibit vascular smooth muscle cells from being transformed into foam cell and could eliminate the atherosclerotic plaque in C57 BL/6J mouse. This experiment is to investigate its mechanism of eliminating plaques in vitro. METHODS: The cultured porcine artery smooth muscle cells incubated with XBP-01 of 0.1 mg/L for 24 hours after preincubated with oxidized low density lipoprotein of 15 mg/L for 72 hours in vitro. The samples were analyzed by fluorescence microscope?confocal microscope system and flow cytometry. RESULTS: Apoptosis was triggered by being incubated with oxidized low density lipoprotein and this process was accelerated additionally by being incubated with XBP-01. CONCLUSION: XBP-01 can be effective in eliminating atherosclerotic plaque by accelerating the process in which oxidized low density lipoprotein induced smooth muscle cell apoptosis

关 键 词：肽类 脂蛋白类 LDL 细胞凋亡 血管平滑肌细胞 VSMC 消斑肽 氯化低密度脂蛋白 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病]