限钠或补钠对充血性心衰大鼠心肌血管紧张素原mRNA表达的影响  

Effects of dietary sodium on the expression of angiotensinogen mRNA in myocardium of rats with congestive heart failure

作　　者：张素珍[1] 徐永[1] 万瑜[1] 杨钢[1] 唐志佼[2] 胡建民[3] 

机构地区：[1]湖北医科大学生理学教研室,湖北武汉430071 [2]湖北医科大学病理学教研室,湖北武汉430071 [3]湖北医科大学实验中心,湖北武汉430071

出　　处：《中国病理生理杂志》2001年第10期938-940,共3页Chinese Journal of Pathophysiology

基　　金：湖北省教委青年科研基金资助项目(No.96C0 5 )

摘　　要：目的 :研究限钠或补钠对充血性心衰大鼠心肌血管紧张素原mRNA表达的影响。方法 :用放射免疫分析法和原位杂交技术分别测定心肌组织血管紧张素Ⅱ (AngⅡ )含量和血管紧张素原 (ANG)mRNA的表达水平。结果 :限钠组血钠显著低于心衰组 (P <0 0 5 ) ,心肌AngⅡ含量、ANGmRNA表达水平及心 /体重比值均显著高于心衰组 (P <0 0 5或P <0 0 1) ;补钠组血钠显著高于心衰组 (P <0 0 5 ) ,与对照组无显著差别 ,其它 3项均与心衰组无显著差别。结论 :心衰后限钠可进一步激活心脏局部肾素 -血管紧张素系统。AIM: This study was designed to investigate the effects of sodium restriction and sodium supplementation on the expression of angiotensinogen mRNA in myocardium of rats with congestive heart failure(CHF).METHODS: Radioimmunassay and in situ hybridization techniques were used to determine the angiotensin Ⅱ(AngⅡ)contents and the expression of angiotensinogen(ANG)mRNA in myocardium, respectively.RESULTS: In sodium restricted group, the plasma sodium was obviously lower than that in CHF group(P<0.05), while the contents of AngⅡ,the expression of ANG mRNA in myocardium and the ratio of heart weight/body weight(HW/BW)were significantly higher than those in CHF group (P<0.05 or P<0.01). It was also found that the AngⅡ, the mRNA contents in rat myocardium and the ratio of HW/BW were not significantly changed in case of sodium supplementation.CONCLUSION: Restriction of sodium intake can further activate the local renin-angiotensin system in CHF heart.

关 键 词：充血性心力衰竭 肾素-血管紧张素系统 血管紧张素原 钠 杂交原位 CHF 大鼠 mRNA 

分 类 号：R541.61[医药卫生—心血管疾病]