孕酮对脑缺血再灌注大鼠纹状体水、钠、钾及钙含量的影响  

Effects of progesterone on water, sodium, potassium and calcium contents in striatum of rats with focal cerebral ischemia/reperfusion

作　　者：李东亮[1] 赵红岗[1] 王东霞[2] 祈英杰[1] 周素凤[1] 席景砖[3] 暴秀梅[3] 

机构地区：[1]新乡医学院生理学教研室,河南新乡453003 [2]新乡医学院三附院检验科,河南新乡453003 [3]新乡医学院预防医学教研室,河南新乡453003

出　　处：《中国病理生理杂志》2001年第10期1012-1015,共4页Chinese Journal of Pathophysiology

基　　金：河南省自然科学基金资助项目 (No .984 0 2 110 0 ) ;河南省医学科技创新人才工程项目 (No.2 4 )

摘　　要：目的 :孕酮 (PROG)已被证明是一种”神经活性甾体” ,本文探讨PROG对缺血脑的神经保护作用。方法 :采用大鼠局灶性脑缺血再灌注模型 ,测定大脑中动脉阻塞 (MCAO) 2h再灌注 2 2h后纹状体水、钠、钾、钙含量。结果 :①I/R和DMSO组MCAO侧纹状体的水、钠、钙明显高于、钾显著低于非MCAO侧 ,I/R和DMSO组之间相比无显著差异 ;②与I/R和DMSO组相比 ,PROG预防及防治组MCAO侧纹状体的水、钠、钙明显减少 ,钾明显增加 (P <0 0 1) ;PROG治疗组则水、钠明显减少 ,但钾和钙无显著差异 (P >0 0 5 ) ;③PROG各组与阳性对照DEXA组相比 ,MCAO侧纹状体水、钠、钙、钾的差别均无显著 (P >0 0 5 )。结论 :PROG可减轻脑缺血 /再灌注大鼠纹状体的损伤。AIM: To explore the neuroprotective action of progesterone(PROG), which has been proved to be a 'neuroactive steroid'. METHODS: The model of focal cerebral ischemia was established in rats by reverserble inserting a nylon thread with a diameter of 0.2 mm into the anterior cerebral artery through the internal carotid artery. The effect of PROG was assessed by determining water,sodium, potassium, and calcium contents in striatum of rats subjected to 2 h ischemia followed by 22 h reperfusion. RESULTS: The water,sodium,and calcium contents of middle cerebral artery occlusion(MCAO) striatum were obviously higher,the potassium content was obviously lower than those of non-MCAO striatum in I/R and DMSO groups,but there was no significant difference between these two groups.Compared with the result in I/R and DMSO groups , water, sodium and calcium contents significantly decreased, but potassium(P<0.01) obviously elevated in MCAO striatum in the PROG-pretreated group and PROG-pre or posttreated group.There was also significant reduction in water and sodium, but not significantly changed in calcium and potassium in PROG-posttreated group. The water,sodium,potassium,and calcium contents of MCAO striatum in each PROG-treated group were not remarkably different in comparision with those in dexamethasone treatment group(P>0.05). CONCLUSION: Treatment with PROG can significantly reduce the striatal injury of rats with cerebral ischmia-reperfusion.

关 键 词：孕酮 脑缺血 钠 钾 钙 再灌注损伤 纹状体 大鼠 

分 类 号：R743.31[医药卫生—神经病学与精神病学]