捕食应激对大鼠行为及空间学习记忆的影响  

Effects of predator stress on emotional behavior and spatial learning and memory of rats

作　　者：王庆松[1] 张建华[2] 

机构地区：[1]成都军区总医院神经精神科 [2]成都军区总医院内分泌科

出　　处：《中华精神科杂志》2001年第3期180-183,共4页Chinese Journal of Psychiatry

基　　金：国家自然科学基金资助项目

摘　　要：目的　建立较理想的创伤后应激障碍 (PTSD)动物实验模型。方法　将 42只雄性Wistar大鼠随机分为单纯捕食应激组 (单纯组 ,n =15 )、加强捕食应激组 (加强组 ,n =15 )和对照组 (n =12 )。单纯组鼠仅与猫无生理伤害性接触 10min(即单纯捕食应激 ) ;加强组鼠则在与猫接触后 1周被再次被置于实验环境中 5min(即加强捕食应激 )。结果　在 1个月的观察时间内 ,加强捕食应激可致实验动物旷场活动性明显减少 [(10 2± 2 4)分 ,P <0 0 5 ],拒俘反应性明显增强 [(2 6 7± 0 5 1)分 ,P <0 0 1]。实验后第 3天 ,加强组鼠Morris水迷宫训练潜伏期明显延长 [(5 8 6± 11 7)s ,P <0 0 1];空间觅向能力测试时在水迷宫的四个象限中无目的漫游。结论　加强捕食应激成功诱发了实验动物较长时间的活动习性改变、警觉水平过高、惊恐行为、环境适应能力下降、躲藏逃避反应等多种行为异常 ,以及短暂空间学习和记忆能力障碍 。Objective To observe the effects of predator stress on emotional behavior and spatial learning and memory of rats in order to establish an practical animal model for posttraumatic stress disorder (PTSD) Methods Forty two male Wistar rats were divided randomly into 3 groups: Group PS for simple predator stress ( n =15), Group PS/CS for intensive predator stress ( n =15), and Group NC for control ( n =12) Non injurious exposure of rats to cat for 10 min for Group PS; and besides that, the experimental rats were put into the same environment except exposure to cat 7 days later for Group PS/CS Results The rats tested one month after intensive predator stress displayed substantial decreases in open field activity (scale: 10 2±2 4, P <0 05), and increases in resistance to capture (scale: 2 67±0 51, P <0 01) The time taken for rat to find the platform (latency) for spatial bias prolonged [(58 6±11 7)s, P <0 05], and the experimental rats swam aimlessly in all four pool quadrants during the probe trial in the Morris watermaze Conclusions The experimental rats by intensive predator stress display the long lasting significant abnormalities of emotional behaviors, such as behavioral habits, anxiety and defense, startle and delayed habituation to startle, and transitory spatial learning and memory dysfunction The rat model of intensive predator stress has ecological validity, it mimics many of the symptoms of PTSD

关 键 词：创伤后应激障碍 行为障碍 学习障碍 记忆障碍 大鼠 PTSD 动物模型 

分 类 号：R749.5[医药卫生—神经病学与精神病学]