人类线粒体tRNAs基因在CagA阳性Hp培养滤液诱导转化的胃上皮细胞中表达  

The expression of several tRNAs human mitochondrial genes in human gastric epithelial cells were transformated by CagA gene positive broth culture filtrates of Helicobacter pylori

作　　者：陈洁平[1] 沈鼎明[2] 杨致邦[3] 

机构地区：[1]第三军医大学附属西南医院肿瘤血液科,重庆400038 [2]重庆医科大学第二临床学院消化内科,重庆400011 [3]重庆医科大学微生物学教研室,重庆400011

出　　处：《第三军医大学学报》2001年第9期1039-1042,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 (3 9870 0 4 6);国家博士后基金资助项目

摘　　要：目的　研究CagA阳性Hp培养滤液对人胃粘膜上皮细胞 (GES 1)作用的机制。方法　将CagA阳性Hp培养滤液诱导恶性转化的GES 1细胞与CagA阴性Hp培养滤液处理的GES 1细胞进行mRNA差异显示 ,以寻找Hp致胃癌发病的相关基因。结果mRNA差异显示片断之一命名为PFN2 ,仅在Hp(CagA+ )培养滤液处理的GES 1细胞中表达 ,斑点杂交结果与之一致。与GeneBank资料序列同源性比较分析提示PFN2与人类线粒体tRNAs同源性 10 0 % ,即为人类线粒体tRNAs基因的一部分。结论　人类线粒体tRNAs基因可能参与了Hp的CagA诱导的胃上皮细胞恶性转化过程。Objective To study the the mechanism of malignant transformation induced by CagA gene positive broth culture filtrates of Helicobacter pylori in human gastric epithelial cells. Methods CagA related gene was researched by the mRNA differential display technique using GES 1 cells which they were treated with CagA gene positive and negative broth culture filtrates of Helicobacter pylori . Results The results of mRNA differential display showed that there are significant differences in geng expression between GES 1 cells treated with CagA gene positive and negative filtrates of Hp. The result of dot blotting analysis also was resemble. An isolated clone showed 100% homology with the tRNAs of human mitochondrial genes. Conclusion The effects of malignant transformation induced by CagA in GES 1 cells, is responsible for the progression of tRNAs of human mitochondrial genes.

关 键 词：幽门螺杆菌 CAGA基因 胃粘膜上皮细胞 线粒体tRNAs基因 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]