幽门螺杆菌CagA抗原性多态性分析  

Analysis of antigenic heterogeneity of CagA of Helicobacter pylori strains

作　　者：周建嫦[1] 张建中[2] 徐采朴[1] 何利华[2] 张茂俊[2] 盛涛[2] 郭浩岩[2] 

机构地区：[1]第三军医大学附属西南医院全军消化专科中心,重庆400038 [2]中国预防医学科学院流行病学微生物学研究所腹泻研究室,北京102206

出　　处：《第三军医大学学报》2001年第9期1049-1050,共2页Journal of Third Military Medical University

摘　　要：目的　了解我国幽门螺杆菌CagA抗原性的多态性 ,为临床血清学诊断CagA阳性菌株感染提供实验依据 ,以及是否存在特定胃十二指肠疾病相关的CagA抗原性型提供线索。方法　采用Westernblots方法 ,比较 38株我国幽门螺杆菌分离株的CagA与 3种不同胃肠疾病分离株兔免疫血清的反应强度。分析不同抗原性类型CagA与疾病的关系。结果　任何一种血清均不能识别所有CagA ,但任一CagA至少可被一种血清识别 ,多数菌株CagA的抗原性与CAPMN6 2接近 ;抗原性与NCTC116 37接近的CagA可能与消化性溃疡相关 ,但不显著。结论　我国不同幽门螺杆菌菌株CagA抗原性存在明显多态性 ,血清学方法诊断CagA阳性菌株感染时应至少使用 3种抗原或抗体 ,不同抗原性类型CagA与胃十二指肠疾病的关系有待进一步研究。Objective To examine the antigenic heterogeneity of CagA of Helicobacter. pylori for identification of CagA positive Hp infection serologically, and determine the existence of gastroduodenal diseases specific CagA antigenic subsets. Methods The antigenic heterogeneity of CagA of different Hp strains was determined by comparing their reactivity to three different types of rabbit sera immunized with CAPM N62, NCTC11637, CAPM Z-2 respectively by Western blots, its' association with different gastroduodenal diseases was analyzed. Results None of the three immune sera could recognize CagA of all isolates, whereas each CagA could be recognized by at least one type of immune serum. The antigenity of CagA of most Chinese Hp strains was similar to that of CAPM N62, those reacted most strongly with serum immunized by NCTC11637 was more likely but not significantly correlated to peptic ulcer. Conclusion CagA of different Chinese Hp strains are in high antigenic heterogeneity, at least three types of CagA antigen or its antibody should be used for determining CagA positive Hp strain infection by serological method. The relationship between different antigenic subsets of CagA and gastroduodenal diseases need further investigation.

关 键 词：幽门螺杆菌 CAGA 抗原多态性 胃癌 胃粘膜萎缩 

分 类 号：R735.2[医药卫生—肿瘤] R573[医药卫生—临床医学]