原花青素抗促癌物诱发H_2O_2释放及脂质过氧化  

Effect of procyanidins on the release of H_2O_2 induced by croton oil and on lipid peroxidation

作　　者：陆茵[1] 孙志广[2] 赵万洲[1] 阮君山[1] 闫新琦[1] 

机构地区：[1]江苏省海洋药物研究开发中心,南京210029 [2]江苏省中医院消化内科,南京210029

出　　处：《中国药理学通报》2001年第5期562-565,共4页Chinese Pharmacological Bulletin

基　　金：中国博士后基金资助项目 ;No [1997] 7号

摘　　要：目的　探讨葡萄原花青素抗氧化和肿瘤化学预防作用机制。方法　以大鼠多形核白细胞 (PMNs)为材料 ,利用酚红氧化原理比色测定了原花青素对巴豆油 (crotonoil)刺激PMNs生成H2 O2 的影响。结果　原花青素能显著性抑制巴豆油刺激PMNs生成H2 O2 ;以原花青素的大鼠含药血清代替反应系统中的药物 ,同样观察到原花青素具有抑制H2 O2 释放的作用 ,该作用在给药后 1h左右最强 ,且具有一定的时效关系和量效关系。对巴豆油诱发的小鼠肝线粒体脂质过氧化 ,原花青素具明显抑制作用 ,能明显提高肝线粒体SOD活力 ,减少MDA生成。AIM To study the anti oxidation and cancer chemoprevention mechanism of procyanidins extracted from Vitis vinifera. METHODS A simple colorimetric method is used for the measurement of H 2O 2 produced by PMNs induced by croton oil. RESULTS It was shown that procyanidins could inhibit PMNs releasing H 2O 2 induced by croton oil. Procyanidins serum of rats also had the same effect. The effect was time and dose dependent. Inhibitory effect of procyanidins on hepatic mitochondria lipid peroxidation induced by croton oil was also observed. Procyanidins could raise the activity of SOD and decrease the level of MDA. CONCLUSION It was concluded that anti oxidation effect of procyanidins was one important mechanism of cancer chemoprevention.

关 键 词：原花青素 VITIS VINIFERA 抗氧化 多形核白细胞 线粒体 抗癌作用 实验研究 

分 类 号：R285.5[医药卫生—中药学] R286.91[医药卫生—中医学]