苯妥英钠对人精子染色体结构畸变的体外实验  

Effects of phenytoin on structural aberration of human sperm chromosomes in vitro

作　　者：蒙华庆[1] 张逢春[1] 高小平[2] 王学峰[3] 李丹[2] 崔晓[2] 沈鼎烈[3] 王增产 

机构地区：[1]重庆医科大学附属第一医院精神科,400016 [2]四川省计划生育科研所 [3]重庆医科大学附属第一医院神经内科,400016 [4]重庆医科大学计划生育系,400016

出　　处：《中华医学遗传学杂志》2001年第4期303-305,共3页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金 (392 70 81 0 )&&

摘　　要：目的　检测苯妥英钠对人精子染色体的诱变效应。方法　应用人精子染色体离体测试系统检测苯妥英钠在 3种不同浓度对人精子染色体的损伤作用 ,并与平阳霉素的阳性对照组和空白对照组比较。结果　苯妥英钠在 10 μg/ ml、2 0 μg/ ml、40 μg/ ml3种浓度的染色体结构畸变精子率和断裂数均较空白对照组高 ,但仅 40 μg/ ml组的增高 ,差异有显著性。Objective: To detect the mutagenic effects of phenytoin on human sperm chromosomes. Methods: The mutagenic effects of phenytoin at 10, 20 and 40 μg/ml were tested by an in vitro testing system of human sperm chromosomes, with leomycin A5 as a positive control and mutagen-free solution as the blank control. Results: The frequencies of sperm with structural chromosomal aberrations and breakages of sperm chromosomes in the groups of 10 μg/ml, 20 μg/ml and 40 μg/ml of phenytoin were all higher than those in the blank control, but only in the group of 40 μg/ml, the elevated frequencies were statistically significant (P < 0.005, P < 0.05). Conclusion: Phenytoin has probably mutagenic potential effect on human sperm cells.

关 键 词：苯妥英钠 精子 染色体结构畸变 实验研究 抗癫痫药 

分 类 号：R965[医药卫生—药理学]