利用蚕豆根尖细胞微核技术检测Cu、As污染的诱变性  

Utilization of micronucleus test in Vicia faba root tips cell to detect the mutability of Cu and As pollution

作　　者：张莉[1] 刘登义[1] 王友保[1] 

机构地区：[1]安徽师范大学生命科学学院,芜湖241000

出　　处：《应用生态学报》2001年第5期777-779,共3页Chinese Journal of Applied Ecology

基　　金：教育部留学回国人员基金;安徽师范大学青年基金资助项目( 2 0 0 0QL37)

摘　　要：应用蚕豆根尖细胞微核技术检测Cu、As及其复合污染的诱变性能 ,计数了蚕豆根尖细胞微核千分率(MCN‰ )和污染指数 (PI)并进行了F检验 .结果表明 ,在一定浓度范围内 (Cu <2 0 0mg·L-1,As <15mg·L-1) ,随着Cu、As浓度的增加 ,MCN‰上升 ;2 4个试验处理组PI均在 2以上 ,各处理组MCN‰有显著差异 ,和对照组有极显著差异 (α <0 .0 1) ,表明Cu、As及其复合处理对蚕豆根尖细胞的分裂活动有明显影响 .The micronucleus test in Vicia faba root tips cell was utilized to detect Cu ,As and their combined pollution.The MCN‰ and PI were determined and the F tes t was used to evaluate the statistical difference in MCN‰ among differente treatments .The results showed that within a certain concentration of Cu 2+ (<200mg · L -1 ) and As 3+ (<15 mg·L -1 ), MCN‰ increased as the concentrat io ns increased.The PI of all the 24 treatment was over 2, and there existed obviou s difference compared with the control (α<0.01abstractThis study shows that Cu, As an d combined pollution had significant effects on cytogenetical toxicity of Vic ia faba root tip cell.MCN‰ is lower than normal level in the complex treatment of Cu and As because of antagonism actions.

关 键 词：蚕豆极尖细胞 CU As 微核检测 诱变性 铜污染 砷污染 

分 类 号：X171.5[环境科学与工程—环境科学] X835