酪酸梭菌与双歧杆菌对肠道致病菌的体外生物拮抗作用  

STUDIES ON THE ANTAGONISM OF CLOSTRIDIUM BUTYRICUM LCL166 STRAIN AND BIFID OBACTERIUM LCL172 STRAIN AGAINST SOME ENTERPATHOGENS IN VITRO

作　　者：张雪平[1] 陆俭[1] 傅思武[1] 肖在滢[1] 罗武英[1] 孟筱琦[1] 

机构地区：[1]兰州生物制品研究所,甘肃兰州730046

出　　处：《中国微生态学杂志》2001年第5期260-262,共3页Chinese Journal of Microecology

摘　　要：目的 :探讨酪酸梭菌和婴儿型双歧杆菌对某些肠道致病菌的拮抗作用。方法 :将酪酸梭菌和婴儿型双歧杆菌单株菌及两株菌混合后分别与几株肠道致病菌混合接种于 GAM液体培养基中进行厌氧培养 ,通过平板菌落计数法计算肠道致病菌的菌数。结果 :经混合培养后肠道致病菌的菌数明显下降。结论 :酪酸梭菌 L CL16 6株和婴儿型双歧杆菌 L CL172株在体外能明显抑制几株肠道致病菌的生长繁殖。The pure culture of Clostridium butyricum LCL166 strain alone ,B ifidobacterium infantis LCL172 (Bif,infantis LCL172)strain alone and mixed cultu res of C butyricum LCL166 and Bif infantis LCL 172 was mixed in vitr o with that o f E Coli O157:H7,Shigella flexneri F2a,Salmonella typhi H034 propot ionally in GA M culture medium respectively During anaerobic incubation,quantities of each en t erpathogens in the mixed cultures were investigate As a result,the amounts of E Coli O157:H7,Shigella flexneri F2a,Salmonella typhi H034 in mixed cult ures of C butyricum LCL 166 and Bif infantis LCL172 were significantly lower than t heir pure culture alone respectively

关 键 词：酪酸梭菌 婴儿型双歧杆菌 生物拮抗作用 肠道致病菌 

分 类 号：R977.9[医药卫生—药品] R378[医药卫生—药学]