天然药物对血链球菌生长和产酸影响的体外研究  

STUDY ON THE EFFECT OF NATURAL MEDICINES ON THE GROWTH AND ACID PRODUCTION OF STREPTOCOCCUS SANGUIS IN VITRO

作　　者：肖悦[1] 刘天佳[1] 黄正蔚[1] 周学东[1] 李罡[1] 

机构地区：[1]四川大学华西口腔医学院,成都610041

出　　处：《中国微生态学杂志》2001年第5期278-280,共3页Chinese Journal of Microecology

基　　金：联合利华公司英国研究中心资助项目

摘　　要：目的 :通过研究不同天然药物对血链球菌生长及产酸的影响 ,为今后筛选出能有效调理口腔菌群生态平衡的药物奠定基础。方法 :选用变形链球菌 SB179作为实验菌株。测定川芎、血藤、五倍子等 11种天然药物的最低抑菌浓度 MIC。再以低于 MIC的 4个浓度配制含药的 TPY液体培养基 ,调定其初始 p H为 7.4,接种血链球菌 ,厌氧培养后测定其终末 p H。结果 :当药物浓度低于或等于 8.0 0 0 mg/ m l时 ,各天然药物对血链球菌的生长均有一定的抑制作用 ,且以五倍子作用较强。槟榔、茶多酚、蜂房、大黄、三七、血藤、白芷对血链球菌的产酸具有一定的抑制能力 ,而黄芩、川芎、五倍子和儿茶没有明显的抑制作用。结论 :槟榔、茶多酚、蜂房、大黄、三七、血藤和白芷对血链球菌的生长和产酸都有一定的抑制作用。Objective:To study the effect of different natura l med icines on the growth and acid production of streprococcus sanguis,as a prepa rati on for screening an effective agent to mediate the balance of oral microflora Methods:Streptococcus sanguis SB179 was chosen as the experi mental bacteria 11 kinds of medicine,such as Rhizoma Chuanxiong,Surgentodoxa cu n eata and Galla Chinensis were extracted by means of maceration,percolation and r eflux extraction First the values of MIC of various extracts were measured Sec on d different experimental medium containing various extracts was prepared The co n centration of the extracts was lower than the MIC of the medicine and the initia l pH of the medium was7 4 Then Streptococcus sanguis was cultrued in the m edium for 48 h,and finally the rest pH was measured Results:Wh e n the concentration of the medicines was lower than or equal to 8 000mg/ml,all k inds of medicine can inhibit the growth of Streptococcus sanguis effectively ,esp ecially Galla Clinensis Radix Notoginseng,Surgentodoxa cuneata,Radixet Rhizoma Rhei,Radix Angelicae Pubescentis,Semen Arecae,Polistes mandarinus,Tea polyphenol s can inhibit he acid production of Streptococcus sanguis effectively,but Radix Scuteilariae,Rhizoma Chuanxiong,Galla Chinensis and Catechu have no preliminary effect on it Conclusion:Semen Arecae,Tea polyphenols,Radi xet Rhizoma Rhei,Radix Notoginseng,Polistes mandarinus,Surgentodoxa cuneata,Rad ix Angelicae Pubescentis can inhibit the growth and the acid production of Str eptococcus sanguis effectively

关 键 词：天然药物 血链球菌 生长 产酸 口腔病原菌 

分 类 号：R780.2[医药卫生—口腔医学] R965.1[医药卫生—临床医学]