西红花酸的体外抗氧化作用的研究  

Antioxidative Activity of Crocetin in vitro

作　　者：龚国清[1] 刘同征[1] 李立文[1] 钱之玉[1] 

机构地区：[1]中国药科大学药理学教研室,南京210009

出　　处：《中国药科大学学报》2001年第4期306-309,共4页Journal of China Pharmaceutical University

摘　　要：目的　探讨西红花提取物中西红花酸的体外抗氧化作用。方法　采用 H2 O2 和 Vc-Fe2 + 两种羟自由基发生系统 ,观察了西红花酸对 H2 O2 系统的羟自由基的清除能力 ,对肝匀浆及肝线粒体的 Vc-Fe2 + 系统引起的脂质过氧化的抑制作用。结果　西红花酸对 H2 O2 系统的羟自由基有较强的清除能力 ,并能抑制肝匀浆自氧化和 Vc-Fe2 + 系统羟自由基引起的脂质过氧化 ,对肝线粒体也有保护作用。AIM\ The effect of crocetin on oxygen free radicals and lipid ox ida tion induced by hydroxyl free radical (HFR) \%in vitro\% were investigated. METHODS\ The scavenging effect of crocetin on ·OH was investigated \%in vi tro\% by means of hydroxyl free radical (HFR) produced by H\-2O\-2. MDA producti o n in liver homogenate induced by autooxidation and Fe\+\{2+\}+Vc free radical ge neration system were measured by TBA methods. RESULTS\ Crocetin posses sed remarkable scavenging effect on ·OH produced by H\-2O\-2. Crocetin could si gnificantly inhabite the lipid peroxidation of liver homogenate induced by auto -oxidation and induced-oxidation. CONCLUSION\ Crocetin possessed an antioxidant activity.

关 键 词：西红花酸 羟自由基 丙二醛 H2O2 维生素C 体外抗氧化作用 实验研究 

分 类 号：R285[医药卫生—中药学]