蛋白激酶C对低氧猪肺动脉平滑肌细胞结构型一氧化氮合酶mRNA表达作用的探讨  

A study of the effect of protein kinase C on the expression of nitric oxide synthase mRNA in pulmonary artery smooth muscle in hypoxia

作　　者：丁毅鹏 徐永健[1] 张珍祥[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院呼吸科,武汉430030

出　　处：《中华结核和呼吸杂志》2001年第10期588-591,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：国家自然科学基金资助 ( 39770 341);原国家教委基金项目 ( 1997 436 );教育部"高等院校骨干教师资助计划"( 2 0 0 0年度 )

摘　　要：目的　探讨蛋白激酶C(PKC)信号传导通道对低氧猪肺动脉平滑肌细胞 (PASMC)结构型一氧化氮合酶 (cNOS)mRNA表达的影响。方法　采用逆转录 聚合酶链 (RT PCR)方法测定了低氧条件下猪PASMC中PKC α和cNOSmRNA表达情况 ,并观察了PKC激活剂和抑制剂对cNOSmRNA表达的影响。结果　低氧 48、72hPKC αmRNA的表达明显升高 (P <0 0 1) ,cNOSmRNA的表达明显降低 (P <0 0 1) ,cNOSmRNA的表达与PKC αmRNA的表达呈显著负相关 (P <0 0 0 1)。PKC激活剂豆蔻酸佛波醇乙酯 (PMA)可使cNOSmRNA在低氧PASMC中的表达进一步降低 ,而PKC抑制剂RO 31 82 2 0的作用相反 ,使cNOSmRNA的表达明显升高 (P <0 0 1)。NO激活剂左旋精氨酸 (L Arg)和抑制剂Nω 硝基 L 精氨酸甲酯 (L NAME)对PKC αmRNA的表达无明显影响。结论　PKC可抑制cNOSmRNA在低氧PASMC中的表达。PKC在低氧性肺动脉高压的形成机制中的作用可能是通过抑制cNOSmRNA在PASMC的表达和对NO的调控来实现的 ,PKC信号通道可能作用在NO的上游 。Objective To investigate the effect of PKC on the expression of NOS mRNA in pulmonary artery smooth muscle cell(PASMC) in hypoxia Methods RT PCR was used to detect the expression of PKC α mRNA in PASMC of procine, and the effect of activator and inhibitor of PKC on the expression of cNOS mRNA in PASMC Results The expression of PKC α mRNA was higher in the PASMC in 48 and 72 h of hypoxia( P <0 01), and the expression of cNOS mRNA was lower( P <0 01) The expression of cNOS mRNA was significantly negatively correlated with the expression of PKC α mRNA( P <0 001) PMA, an agonist of PKC could reduce the expression of cNOS mNRA in hypoxia, while RO 31 8220, an inhibitor of PKC could inhibit it and enhance the expression of NOS mRNA( P <0 01) The activator of NO, L Arg and inhibitor, L NAME had no effect on expression of PKC α mRNA Conclusion The PKC can inhibit the expression of cNOS mRNA in PASMC in hypoxia The effect of PKC in hypoxic pulmonary hypertension may be made by inhibiting the expression of cNOS mRNA and modulating NO The site of the effect of PKC may be in upstream of NO The mechanism of it needs to be studied further

关 键 词：低氧 肺动脉平滑肌细胞 蛋白激酶C 一氧化氮合酶 MRNA 肺动脉高压 

分 类 号：R362[医药卫生—病理学]