肺炎衣原体肺炎小鼠模型的建立与实验研究  

Establishment and experimental study on mouse model ofChlamydia pneumoniaepneumonitis

作　　者：施毅[1] 印洁[1] 詹化文[2] 冯根宝[3] 苏欣[1] 夏锡荣[1] 周晓军[2] 申萍[1] 胡兰萍[3] 朱美英[1] 

机构地区：[1]南京军区南京总医院呼吸科,南京210002 [2]南京军区南京总医院病理科,南京210002 [3]南京军区南京总医院内科实验室,南京210002

出　　处：《中华结核和呼吸杂志》2001年第10期592-595,T001,共5页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：江苏省科委自然科学基金应用基础研究计划资助(BJ980 6 5 )

摘　　要：目的　通过复制小鼠肺炎衣原体感染模型 ,对肺炎衣原体肺炎的发病机制进行初步的探讨。方法　以肺炎衣原体鼻内或静脉接种Icr小鼠 ,通过不同时点 (6 0d内 )处死动物 ,观察其肺部的病理改变。结果　鼻内接种肺炎衣原体后 ,Icr小鼠可以产生肺部感染 ,特征性病理改变为斑片状间质性肺炎 ,早期 (7d内 )病变较重 ,以嗜中性粒细胞浸润为主 ,并伴有泡沫细胞堆积 ;后期 (14d以后 )病变开始减轻 ,以嗜中性粒细胞和淋巴细胞混合浸润为主 ,并逐渐转为以淋巴细胞浸润为主。静脉接种肺炎衣原体后的肺部改变与鼻内接种类似 ,但病变程度要轻得多 ,范围也要小得多 ,恢复正常的时间也较快 ,两者差异在发病早期更为明显。以聚合酶链反应 (PCR)法在接种后的肺组织中间歇性检测到肺炎衣原体DNA。被接种的小鼠可以产生血清IgG抗体。结论　给Icr小鼠接种肺炎衣原体可以产生以间质性肺炎为特征的肺部感染 。Objective To evaluate mice as experimental animals for Chlamydia pneumoniae ( C. Pneumoniae ) infection and investigate the pathogenesis of C. pneumoniae pneumonitis. Methods The Icr mice were inoculated with C. pneumoniae , strain CWL 029, by the intranasal or intravenous routes. After a single inoculation, mice were killed on the 1st,3 rd,7 th,15 th,21 th,28 th and 60 th day separately. The pathological changes of lung tissue were analyzed.Results The Icr mice were shown to be susceptible to C. pneumoniae . Inoculation of mice with C. pneumoniae induced a prolonged course of lung infection, as demonstrated by persistence of lung pathology (60 days). In the intranasal inoculation of mice, the lung pathology was characterized by patchy interstitial pneumonitis with predominately neutrophil leukocyte infiltration in the early(7 days) and lyphocytes infiltration in the later stages(14 days later) of infection. After iv inoculation, a similarly interstitial pneumonitis was seen, but it was milder and more patchy, especially in the early stage. C. pneumoniae DNA was detected by polymerase chain reaction(PCR) intermittently in the lung tissue. Inoculated mice developed serum IgG antibody responses.Conclusions The Icr mice were shown to be susceptible to C. pneumoniae and the pulmonary infection was characterized by interstitial pneumonitis, especially in mice of intranasal inoculation.

关 键 词：肺炎衣原体 呼吸道感染 动物感染 病理改变 实验研究 肺炎 

分 类 号：R-332[医药卫生]