脑星形细胞瘤Fas和FasL的表达及细胞凋亡与肿瘤恶性程度相互关系的初步探讨  

Expression of Fas and FasL Gene in Astrocytomas and Relationship between Apoptosis and Malignant Grade of Astrocytomas

作　　者：钟志光[1] 刘安民[1] 邓跃飞[1] 谭平国[1] 蔡望青[1] 李方成[1] 钟伟健[1] 陈由芝[1] 林吉惠[1] 

机构地区：[1]中山医科大学孙逸仙纪念医院神经外科,广东广州510120

出　　处：《癌症》2001年第9期932-935,共4页Chinese Journal of Cancer

摘　　要：目的:对脑星形细胞瘤Fas和FasL的表达水平及细胞凋亡与肿瘤恶性程度之间的关系进行初步探讨。方法:对41例脑星形细胞瘤标本采用免疫组化法(S-P法)检测Fas和FasL的表达率及表达强度,采用TUNEL法检测凋亡指数。结果:本组星形细胞瘤Fas和FasL的表达率分别为65.9%和78.0%;Fas和FasL的表达率与其病理分级有关(χ2分别为17.552和10.749,P均<0.05);Fas和FasL的表达强度也与其病理分级呈负相关(r分别为-0.660和-0.506,P均<0.01)。本组星形细胞瘤的凋亡指数为0.51～1.57,凋亡指数与肿瘤病理分级有关(F=18.828,P<0.05)。结论:本组大部分星形细胞瘤均有Fas和FasL的表达,表达率和表达强度均随肿瘤恶性程度增高而明显下降;星形细胞瘤的凋亡指数随肿瘤恶性程度增高而明显增高。Objective: To investigate the expression of Fas and FasL in astrocytomas and the relationship between apoptosis and malignant grade of astrocytomas. Methods: Expressions of Fas and FasL were determined by utilizing S P immunohistochemical staining, and apoptosis cells were labeled by TUNEL technique for 41 astrocytomas specimens. Results: Expression rates of Fas and FasL were 65.9% and 78.0%, respectively. Expression rates of Fas and FasL were negatively related to malignant grade of astrocytomas (χ2=17.552 and 10.749, respectively; P< 0.05), and expression intensities of Fas and FasL were also negatively associated with malignant grade of astrocytomas (r=-0.660 and -0.506,respectively;P< 0.01). Apoptosis indexes were 0.51-1.57,which were positively related to malignant grade of astrocytomas (F=18.828,P< 0.05). Conclusion: Expressions of Fas and FasL were found in most of astrocytomas, and expression rates and expression intensities were decreased significantly with increasing of malignant grade of astrocytomas. Apoptosis indexes were increased significantly with increasing of the malignant grade of astrocytomas.

关 键 词：脑星形细胞瘤 FAS FASL 细胞凋亡 肿瘤恶性程度 

分 类 号：R739.41[医药卫生—肿瘤]