一氧化氮合酶在膀胱癌中的表达及其与肿瘤血管形成的关系  

Nitric Oxide Synthase Expression in Human Bladder Cancerand Their Relationship with Angiogenesis

作　　者：林震[1] 陈仕平[1] 叶传忠[1] 朱绍兴[1] 

机构地区：[1]福建医科大学附属协和医院泌尿外科,福建福州350001

出　　处：《癌症》2001年第9期940-942,共3页Chinese Journal of Cancer

基　　金：福建省卫生厅青年基金资助项目(闽卫教[2000]355号)

摘　　要：目的:探讨一氧化氮(NO)在膀胱肿瘤中的作用及其与肿瘤血管形成的关系。方法:采用免疫组化方法对58例膀胱移行细胞癌及14例正常膀胱粘膜进行一氧化氮合酶(NOsynthese,NOS)抗体染色和微血管密度计数(microvessaldensity,MVD),观察NOS表达结果与肿瘤生物学特性及其肿瘤血管形成之间的相关性。结果:诱导型NOS(iNOS)在膀胱癌组织中的阳性表达率为81.0%(47/58),而正常膀胱粘膜则无表达,其表达程度与肿瘤分级、分期无关(P>0.05);iNOS表达阳性组和阴性组的MVD分别为每个高倍视野39.3±19.5和29.3±10.5,即iNOS的表达程度与肿瘤的微血管密度成正比(P<0.01);而内皮型NOS(eNOS)在膀胱肿瘤和正常膀胱粘膜的血管内皮细胞都有明显的表达;而在移行上皮细胞中则未见表达。结论:iNOS在膀胱癌中表达,在正常膀胱粘膜中则未见表达,提示iNOS和eNOS在膀胱移行细胞癌的血管形成中都起重要的作用。Objective: Nitric oxide (NO) is synthesized by the enzyme family of NO synthases (NOS) and plays an important role in tumor growth and angiogenesis. The authors evaluated NOS expression in the bladder tissue from the patients with transitional cell carcinoma (TCC) of the bladder and its relationship to angiogenesis. Methods: Bladder carcinoma tissue specimens were procured from 58 patients with TCC and 14 cases of benign tissue as contrast group. NOS immunohistochemistry were performed on all tissue specimens and microvessal density (MVD) was counted by endothelial cells immunostained. Results: Inducible NOS specific proteins were found in 47 of 58 bladder cancer specimens but not in control bladder tissue. The malignant cells and inflammatory cells within the carcinomas were highly (inducible NOS, iNOS)iNOS positive, specimens of bladder mucosa outside the malignant regions showed only a weak positive iNOS immunostaining. The endothelial cells in both normal urothlium and tumor tissue showed a highly positive (endothelial NOS, iNOS) immunostaining but its immunoreaction was not detected in either malignant or benign epithelium. MVD was 39.3±19.5/HP and 29.3±10.5/HP in TCC positive and negative for iNOS, respectively (P< 0.01). A correlation between iNOS immunoreactivity and tumor grade in bladder carcinoma could not be verified. Conclusions: Bladder carcinoma tissue had a high iNOS content; while benign tissue did not. NO generation from iNOS in the malignant epithelium and from eNOS in tumor stroma play an important role in tumor angiogenesis.

关 键 词：膀胱肿瘤 一氧化氮合酶 肿瘤血管形成 免疫组织化学 

分 类 号：R737.14[医药卫生—肿瘤]