人端粒酶催化亚单位及其相关蛋白在骨肿瘤中的表达  

Expression of Human Telomerase Catalytic Subunit(hTERT) and Human Telomerase Associated Protein(TP1) in Bone Tumors

作　　者：陈剑琳[1] 丘钜世[1] 傅宇阳[2] 

机构地区：[1]中山医科大学病理学教研室,广东广州510089 [2]中山医科大学附属第一医院外科,广东广州510080

出　　处：《癌症》2001年第9期943-947,共5页Chinese Journal of Cancer

摘　　要：目的:研究骨肿瘤中端粒酶相关基因中,人端粒酶催化亚单位(humantelomerasecatalyticsubunit,hTERT)和人端粒酶相关蛋白(humantelomeraseassociatedprotein,TP1)两个重要成分的表达及对评估肿瘤良、恶性的意义。方法:提取50例手术切除骨肿瘤组织、2种人成骨肉瘤细胞系Saos-2、OS732和原代培养人成骨细胞的总RNA。用一步RT-PCR方法检测hTERT和TP1mRNA的表达。结果:所有骨肿瘤标本及细胞系均检测到TP1的表达。hTERT在17例良性骨肿瘤、33例骨肉瘤、2种人成骨肉瘤细胞系和人成骨细胞的表达率分别为64.7%、57.6%、0%和0%。良、恶性骨肿瘤间hTERT表达的差异不具有显著性(P>0.05),不同分化程度骨肉瘤间hTERT表达的差异也不具有显著性(P>0.05)。结论:一步RT-PCR是检测端粒酶亚单位的有效方法。hTERT和TP1表达水平的上调在骨肿瘤恶性进展中可能不起主要作用,在骨肿瘤发展和维持中可能有替代性(ALT)机制导致细胞永生化。Objective: To investigate the expression level of two major components of the telomerase associated genes: human telomerase catalytic subunit (hTERT) and human telomerase associated protein (TP1) in bone tumoss and its significance in evaluating tumor malignancy. Methods: Total RNA was obtained from 50 surgical bone tumors, 2 human osteoblastic osteosarcoma cell lines (Saos 2 and OS732) and a primary human osteoblast culture. The hTERT and TP1 mRNA were analyzed using method of one step reverse transcriptase polymerase chain reaction (RT PCR) . Results: The TP1 expression was found in all samples examined. In contrast, hTERT expression was detected in 11 of 17 benign bone tumors (64.7%) and in 19 of 33 skeletal sarcomas (57.6%). No hTERT was expressed in 2 human osteosarcoma cell lines, nor in the human primary osteoblast (0%). There was no remarkable difference in hTERT expression between benign and malignant bone tumors (P >0.05). Meanwhile, no notable difference in hTERT expression had been observed among different differentiated types of skeletal sarcomas (P >0.05). Conclusions: One step RT PCR is a powerful method for detecting telomerase subunits. The up regulation of hTERT and TP1 expression may not play a critical role in bone tumor malignant progression. There might be an alternative mechanism for cell immortalization, yet to be determined, in the development and maintenance of bone tumors.

关 键 词：端粒酶 端粒酶催化亚单位 端粒酶相关蛋白 骨肿瘤 

分 类 号：R738.1[医药卫生—肿瘤]