胃癌及淋巴结转移灶中CD44v5、CD44v6和上皮钙粘素的表达及意义  

Significance and Expression of CD44v5,CD44v6, and E-cadherin in Patientswith Gastric Carcinomas and Their Lymph-Node Metastases

作　　者：姜汉国[1] 唐慰萍[1] 廖新波[1] 曹军[1] 吴冬梅[1] 林虹[1] 

机构地区：[1]广东医学院病理教研室,广东湛江524023

出　　处：《癌症》2001年第9期965-969,共5页Chinese Journal of Cancer

摘　　要：目的:探讨CD44v5、CD44v6和上皮钙粘素(epithelialcadherin,E-cd)在胃腺癌及淋巴结转移灶中的表达及其意义。方法:应用免疫组化SABC法检测20例正常胃粘膜、20例胃粘膜上皮异形增生组织、185例胃癌原发灶及117例淋巴结转移灶中CD44v5、CD44v6和E-cd表达。结果:CD44v5、CD44v6和E-cd在胃粘膜异型增生的阳性表达率依次为10.0%(2/20)、20.0%(4/20)和85.0%(17/20)。胃癌原发灶及淋巴结转移灶中其阳性表达率依次分别为43.8%(84/185)、66.5%(123/185)、53.0%(98/185)和71.8%(84/117)、87.2%(102/117)、26.5%(31/117)。三者之间有显著差异(P<0.01)。CD44v5阳性表达率与胃癌生长方式、组织学类型、血管淋巴管内浸润及TNM分期密切相关,而CD44v6同上述因素在统计学上则无显著性差异(P>0.05)。E-cd阳性表达与胃癌生长方式、组织学类型及TNM分期显著相关。早期肠型胃癌CD44v6阳性表达率显著高于弥漫型(P<0.05)。而在进展期胃癌,CD44v5的阳性表达则同弥漫性胃癌显著相关(P<0.05),E-cd的阳性表达率下降主要见于弥漫型胃癌。结论:CD44v5、CD44v6和E-cd阳性表达同胃癌分化及生物学行为密切相关,是预测其侵袭转移的有价值指标。同时,此结果也支持胃癌两种不同起源的假说。Objective: To investigate the significance and the expression of CD44v5,CD44v6, and epithelial cadherin (E-cd) in gastric primary carcinomas and metastatic lymphnodes . Methods: Immunohistochemical method was used to examine CD44v5, CD44v6, and E-cd expression in 20 normal gastric mucosa tissues, 20 gastric epithelial dysplasia, 185 gastric adenocarcinomas, and 117 metastatic adenocarcinoma lymph nodes. Results: The positive expression rates of CD44v5, CD44v6, and E-cd in gastric epithelial dysplasia were 10.0%(2/20), 20.0%(4/20),and 85.0%(17/20), respectively. In gastric primary adenocarcinomas and metastatic lymphnodes,expression rates were 43.8%(84/185), 66.5%(123/185), and 53.0%(98/185); and 71.8%(84/117), 87.2%(102/117), and 26.5%( 31/117), respectively. There was significant difference among the three groups (P< 0.01). The positive expression of CD44v5 was closely correlated with differentiation, histological type, TNM categories,lymph node involvement, and the presence of blood, and lymphatic vessel invasion, whereas such a correlation could not be found for the CD44v6. The positive expression of E-cd was closely correlated with differentiation, histological type, and(TNM) categories. In early gastric carcinomas, the expression of CD44v6 in intestinal-type was much higher than that of diffuse-type(P< 0.05) . The expressions of CD44v5 was closely linked to diffuse-type advanced gastric carcinomas. The expression reduction of E-cd was closely related to diffuse-type early and advanced gastric carcinomas. Conclusion: CD44v5, CD44v6, and E-cd might be the valuable markers in assessing biological behavior, invasion, and metastatic potential of gastric carcinomas. The results also support the hypothesis of different origins for the two tumor types.

关 键 词：胃肿瘤 淋巴结转移 CD44V5 CD44V6 上皮性钙粘素 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤] R73－37[医药卫生—临床医学]