逆转录法制备地高辛标记的食管癌cDNA探针  

Preparation of esophageal carcinoma cDNA probe labeled with digoxigenin by reverse transcription reaction

作　　者：陈东[1] 陈奎生[1] 张云汉[1] 殷智榕[1] 高冬玲[1] 李文才[1] 宋一民[1] 马长路[1] 

机构地区：[1]河南医科大学第一附属医院病理科,河南省肿瘤病理重点实验室,郑州450052

出　　处：《河南医科大学学报》2001年第5期524-526,共3页Journal of Henan Medical University

基　　金：河南省科学技术发展计划资助项目 ( 97 6 2 0 )

摘　　要：目的 :制备食管癌cDNA探针用于ReverseNorthernBlot筛选差异基因片段。方法 :提取食管癌组织总RNA ,通过逆转录反应 ,用地高辛标记为cDNA探针。结果 :所制备的探针灵敏度高达 0 .0 1ng/L。结论 :用该方法制备cDNA探针快速、经济 ,所需模板RNA量少 ,可用于大规模初筛差异基因片段的研究。Aim: To prepare the esophageal carcinoma cDNA probe for reverse northern blot in screening the differential gene fragments.Method: The cDNA probe of esophageal cancer tissue was labeled with digoxigenin by reverse transcription reaction, and applied in reverse northern blot to initially screen the retrieved differential cDNA fragments. Results: Sensitivity of the probe was as high as 0.01 ng/L. Conclusions: It is a rapid economical technique to prepare cDNA probe, and might be used in large scale screening of the differential fragments.

关 键 词：逆转录反应 探针 地高辛 食管肿瘤 CDNA 

分 类 号：R735.1[医药卫生—肿瘤]