神经生长因子对红藻氨酸致痫大鼠海马神经细胞凋亡和野生型P^(53)表达的影响  

Effects of nerve growth factor on the neuronal apoptosis and wild-type P^(53) protein expression in the hippocampus of the rats with kainic\|acid\|induced seizures

作　　者：柳忠兰[1] 冯昱[1] 刘冰[2] 

机构地区：[1]中国医科大学第一临床学院神经内科,辽宁沈阳110001 [2]中山医科大学第一附属医院神经内科,广东广州510089

出　　处：《中风与神经疾病杂志》2001年第4期207-209,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的　探讨外源性神经生长因子 ( NGF)对癫痫发作大鼠海马神经细胞凋亡与野生型 P5 3 ( wt P5 3 )蛋白表达有无抑制作用。方法　采用 KA诱导癫痫发作大鼠模型。以 TUNEL方法标记 DNA片段 ,原位检测凋亡细胞 ;免疫组化 SP法检测 wt P5 3 蛋白。观察大鼠癫痫发作 4 0 h(注射 KA4 8h)后其海马 CA1神经细胞凋亡和 wt P5 3 蛋白表达情况及外源性 NGF对它们的影响。结果　大鼠急性癫痫发作后 ,其凋亡细胞和 wt P5 3 蛋白表达均明显增加 ,wt P5 3 蛋白表达与细胞凋亡呈正相关 ( r =0 .83 9,P <0 .0 1 ) ;给予 NGF,凋亡细胞数及 wt P5 3 表达均显著性降低 ( P<0 .0 1 )。结论　外源性 NGF对癫痫所致神经细胞凋亡有抑制作用 ,其抑制凋亡的分子机制可能是通过抑制 wt P5 3蛋白表达而实现的。Objective To explore whether exogenous nerve growth factor (NGF) might inhibit the hippocampal neuronal apoptosis and wild\|type P 53 (wtP 53 ) protein expression induced by acute seizures or not Methods The model of kainic acid induced epilepsy was used,apoptotic cell death was identified with the terminal deoxynucleotidyl transferase mediated dUTP biotin in situ nick end labeling (TUNEL) technique to detect DNA fragmentation and wtP 53 protein expression was determined with immunohistochemistry(SP) After the seizures stopped for 40 h (kainic acid injections for 48 h),effects of NGF on the neuronal apoptosis and wtP 53 protein expression in the hippocampus of the rats were observed Results After acute seizures,apoptotic neurons and wtP 53 \|protein neurons all increased significantly Correlation analysis showed that neuronal apoptosis had positive correlation with wtP 53 protein expression (r=0 839,P<0 01) Injected NGF into the cerebral ventricle,the rats had less apoptosis neurons and wtP 53 protein neurons than those not injected NGF and killed at the same time (P<0 01) Conclusion Exogenous NGF can inhibit the hippocampal neuronal apoptosis induced by acute seizures,molecular mechanisms of which may be attributed to wtP 53 expression inhibited

关 键 词：神经生长因子 癫痫 大鼠 野生型P^53 细胞凋亡 红藻氨酸 NGF 

分 类 号：R742.1[医药卫生—神经病学与精神病学]