贝美格致痫大鼠皮层、海马γ-氨基丁酸和谷氨酸免疫反应细胞的改变  

Alteration of GABA and Glu immunoreactive cells of cerebral cortex and hippocampus in seizures rats induced by Bemegride

作　　者：王群[1] 阮旭中[2] 李震中[2] 郑乃智[2] 张苏明[2] 

机构地区：[1]北京天坛医院神经内科,北京100050 [2]华中科技大学同济医学院同济医院神经内科,湖北武汉430030

出　　处：《中风与神经疾病杂志》2001年第4期215-217,共3页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金重点项目资助 ( 3 93 3 0 2 1 0 )

摘　　要：目的　γ-氨基丁酸 ( GABA)和谷氨酸 ( Glu)是脑内重要的抑制性和兴奋性神经递质 ,二者与癫痫发作密切相关 ,为了阐明癫痫发病的病理生理机制。方法　我们采用免疫细胞化学双 PAP法 ,观察了贝美格( Bemegride,Be)腹腔致痫大鼠顶叶大脑皮层、海马 CA1、CA3 、齿状回 Glu和 GABA免疫反应细胞的改变。结果　图像分析结果显示 :Be致痫组大脑皮层、海马 Glu免疫反应平均阳性细胞数及光密度较正常对照组明显增加 ( P<0 .0 1 ) ;GABA细胞数及光密度减少 ( P <0 .0 1 )。结论　提示贝美格致痫作用与调节脑内 GABA和 Glu系统的兴奋性有关。Objective Neurotransmitters,mainly excitatory and inhibitory amino acids were implicated in epileptogenesis.To clarify the physiopathological mechanisms of epilepsy induced by Bemegride(Be).Method We observed the alterations of γ aminobuteric acid (GABA) and glutamate(Glu) immunoreactive(IR) cells of cerebral cortex and hippocampus in seizure rats after acute Be administration(25mg/kg,intraperitoneally) by means of immunocytochemical method.Results The experimental results demonstrated that the number and density of GABA IR cells of cerebrocortex and hippocampus in convulsive rats induced by Be decreased while those of Glu IR cells increased(P<0.01).Conclusion These findings suggest that Be enhance epileptic activity via regulating the excitability of GABA ergic and Glutamagic systems in brain.

关 键 词：贝美格 癫痫 Γ-氨基丁酸 谷氨酸 皮层 海马 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]