甲基单胞菌整细胞催化环氧丙烷的连续生物合成  

Continuous Biosynthesis of Epoxypropane Using Whole Cells of Methylomonas sp. GYJ3

作　　者：辛嘉英[1] 崔俊儒[1] 陈建波[1] 李树本[1] 夏春谷[1] 

机构地区：[1]中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室,兰州730000

出　　处：《催化学报》2001年第5期457-460,共4页

基　　金：国家自然科学基金重点项目 ( 2 99330 40 ) ;国家重点基础研究规划项目

摘　　要：利用含有甲烷单加氧酶 (MMO)的甲基单胞菌Methylomonassp .GYJ3整细胞催化丙烯环氧化制取环氧丙烷时 ,辅酶NADH的消耗和产物抑制是反应难以连续进行的主要原因 .为解决这些问题 ,通过批式反应考察了丙烯 甲烷共氧化反应合成环氧丙烷的可能性 ,发现反应气体中甲烷含量为 30 %时环氧丙烷的产量较高 .在搅拌式生物反应器中 ,通过最佳配比的混合反应气体的连续循环将产物环氧丙烷抽提出来 ,从而克服了产物抑制 .该生物反应器最初的环氧丙烷日产量为 2 6 8μmol,连续操作 12d后 ,MMO仍保留 96The whole cells of Methylomonas sp. GYJ3 containing methane monooxygenase (MMO) were used for the epoxypropane biosynthesis from propene. The continuous production of epoxypropane was difficult to proceed for a long time because of the coenzyme NADH exhaustion and the product inhibition. In order to solve these problems, propene-mathane co-oxidation was employed to regenerate NADH. Batch experiments gave maximum production of epoxypropane when methane content in the reaction gas mixture was 30%. In continuous biosynthesis experiments, mixed gaseous substrates of propene, methane and oxygen with an optimum volume ratio of 1:1.5:2.5 were continuously circulated through the stirred tank bioreactor to remove epoxypropane from the cell suspension. An initial epoxypropane productivity of 268 mu mol/d was obtained. The bioreactor can be operated continuously for 12 days without obvious loss of epoxypropane productivity, and more than 96% of the initial MMO activity was retained.

关 键 词：甲基单胞菌 甲烷单加氧酶 丙烯 环氧化 环氧丙烷 生物合成 甲烷 共氧化 酶催化 

分 类 号：O623[理学—有机化学]