RT-PCR测定乳癌及腋窝淋巴结微量组织MUC1及GST-π基因的表达  

Detection of MUC1 and GST-π Gene by means of RT-PCR in Axillary Lymph Nodes Taken from Breast Cancer Patients

作　　者：李继梅[1] 李春海[2] 王秦秦[1] 唐睿珠[1] 孙丽亚[2] 郝萍[1] 姜平[1] 

机构地区：[1]明医学院第一附属医院,昆650031 [2]军事医学科学院肿瘤分子生物学研究室

出　　处：《肿瘤防治研究》2001年第5期351-353,共3页Cancer Research on Prevention and Treatment

基　　金：云南省自然科学基金资助项目 (96 C0 77Q)

摘　　要：目的　研究 MUC1及 GST-π m RNA在乳癌腋窝清扫淋巴结中表达与乳癌发生、发展、预后转归及临床耐药的相关性。方法　用 RT- PCR技术检测 2 0例乳腺癌病人的 1 0 8个 HE染色阴性淋巴结 MUC1及 GST-π m RNA的表达。结果　 MUC1及 GST-π m RNA在 HE染色阴性淋巴结中阳性检出率分别为 66%及 39% ,在乳癌组织、HE阳性淋巴结及正常乳腺组织中阳性率分别为 1 0 0 % /88%、1 0 0 % /71 %及 1 0 0 % /0。结论　HE阴性淋巴结中出现 MUC1阳性表达表明存在肿瘤微转移灶 ,MUC1 m RNA检测可提高淋巴结微转移诊断率 ;GST- π在肿瘤启动和促进阶段可表达 ,是一种重要的乳腺癌生物学标志物 ;乳癌组织中耐药性普遍存在 ;MUC1及 GST- π m R-NA在腋淋巴结的表达可能是预后不良的标志 ,二者联合检测对乳癌早期诊断及预后判断有重要意义。Objective To study MUC1 and GST π mRNA expression in axillary lymph nodes and its clinical significance.Methods Expression of both MUC1 mRNA and GST π mRNA was detected by RT PCR in 108 histologically negative lymph nodes obtained from 20 breast cancer patients.Results Of 108 lymph nodes,66%/39% were found to express MUC1 mRNA/GST π mRNA.And the positive rates of MUC1 and GST π mRNA in cancer tissues,HE positive lymph nodes and normal breast tissue are 100%/87%,100%/80%,100%/0,respectively.Conclusion Expression of MUC1 mRNA in histologically negative lymph nodes suggests the presence of breast cancer micrometastases;GST π ,whose expression is detected as cancer occurring and developing,can be used as a breast tumor marker;Drug resistance exists in almost all the breast cancer,The expression of MUC1 mRNA and GST π mRNA in lymphnodes may be a indication of poor prognosis.The detection of all these two genes together by RT PCR has higher sensitivity and significance.

关 键 词：MUC1粘蛋白 人胎盘型谷胱甘肽转移酶 RT-PCR 乳腺肿瘤 乳癌 诊断 

分 类 号：R737.9[医药卫生—肿瘤] R730.43[医药卫生—临床医学]