鸽左视网膜摘除视盖的人TMEFF2同源基因差异表达  

Differential Expression of a Gene Fragment Homologous to Human TMEFF2 in Right and Left Optic Tecta of Pigeons with the Removal of Left Retina

作　　者：吕立夏[1] 徐磊[1] 杨翠香[1] 李学礼[1] 王尧[1] 

机构地区：[1]同济大学医学院生物化学教研室,上海200070

出　　处：《同济大学学报（医学版）》2001年第5期1-3,共3页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金资助项目 ( 39770 2 44)

摘　　要：目的　研究在左视网膜摘除后鸽与人TMEFF 2同源基因在左右视盖的差异表达。方法　从本室构建的左视盖消减基因差异表达的文库得到一基因片段 ,该片段与人TMEFF 2高度同源。采用逆转录PCR和RNA点杂交方法研究该片段在左视网膜摘除后鸽左右视盖的差异表达。结果　RT PCR和RNA点杂交表明该片段在左视盖高表达。结论　TMEFF 2为中脑神经元和海马神经元存活因子 。Objective To study the differential expression of gene fragment in right and left optic tecta of pigeons with removal of left retina.Methods An expressed sequenced tag (EST)from differentially expressed library for left tecta of pigeon with left retina removal showed high homology with homo sapiens TMEFF2. RT PCR and RNA dot blotting were performed to analyze its expression in right and left optic tecta.Results The EST had a higher expression level in left optic tecta.Conclusion TMEFF2 is a novel survival factor for hippocampal and mesencephalic neurons. It is suggested that EST might play an important role in visual development.

关 键 词：抑制消减杂交 基因表达 视盖 鸽 视网膜摘除 脑功能 TMEFF2 同源基因 

分 类 号：R338[医药卫生—人体生理学] Q781[医药卫生—基础医学]