人工角膜在兔角膜内的生物学反应  

Keratoprosthesis design and biological response to a synthetic cornea

作　　者：吴欣怡[1] V.Trinkaus-Randall A.Tsuk 

机构地区：[1]山东大学齐鲁医院眼科,济南250012 [2]Boston University Medical Center,USA

出　　处：《中华眼科杂志》2001年第6期462-464,T004,共4页Chinese Journal of Ophthalmology

基　　金：国家自然科学基金资助项目 ( 39970 779) ;卫生部科研基金资助项目 ( 98 2 15 8) ;山东省科委基金资助项目 ( 1999BBCJA3)

摘　　要：目的　评价 3种不同直径人工角膜在动物体内应用的生物学反应。方法　将 35只兔随机分为Ⅰ组 (11只 )、Ⅱ组 (12只 )、Ⅲ组 (12只 ) ,分别植入 3种不同直径人工角膜 (4 5～ 7 0mm、7 0～ 9 0mm、4 5～ 6 0mm) ,术前将人工角膜进行纤维细胞组织培养 ,光学中心前表面经rf argonplasma处理后植入兔眼 ,观察术后人工角膜在兔角膜中的存留 ,观察时间 >6个月 ;并分别于术后 2、3、4及 6周摘取兔植入人工角膜行组织及病理学检查。结果　术后 2周成纤维细胞可沉积在人工角膜周边多孔支架内 ;3周时Ⅲ组角膜上皮细胞位于人工角膜光学中心 ,Ⅰ、Ⅱ组未见细胞生长。 4周时人工角膜内可检测出胶原蛋白沉积。前房稳定 ,未见房水渗漏。术后 6周时人工角膜的周边支架中检测出heparansulfate(HS)及碱性成纤维细胞生长因子 ,后者存在于周边支架和宿主角膜的交接处。结论　体外人工角膜组织培养可增加人工角膜周边支架的纤维化 ,稳定光学中心 ;人工角膜经rf argonplasma处理可增加材料的组织相容性 ;兔角膜缘上皮细胞可迁徙至人工角膜光学中心的前表面。Objective To evaluate the biological response to three different designs of synthetic cornea in vivo. Methods All devices consist of a transparent center and a porous blown periphery. Thirty-five devices were implanted into 35 rabbits′ corneas and followed up to more than 6 months. Rabbit corneal stromal cells were inoculated onto the anterior surface of the synthetic cornea and cultured in vitro for one week before the operation. The anterior surface of the optic center hydrogel was modified with radio frequency (rf)-argon plasma treatment. Results Fibroplasia occurred 2 weeks after surgery and collagen was detected by 28 days. The anterior chamber was normal without detectable leakage of aqueous humor. Basic fibroblast growth factor (bFGF) was detected at the interface between the device and the tissue by 42 days. Conclusions It is demonstrated that rabbit limbal epithelial cells can migrate onto the synthetic cornea in vivo.

关 键 词：人工角膜 碱性成纤维细胞生长因子 组织相容性 生物学反应 兔 

分 类 号：R779.6[医药卫生—眼科]