人原发性肝内胆管细胞癌中HCV基因及其抗原表达的意义  

Significance of HCV gene and its antigen expression in human primary intrahepatic cholangiocarcinoma

作　　者：王文亮[1] 王春杰[1] 王冰枫[1] 

机构地区：[1]中国人民解放军第四军医大学肿瘤研究所,陕西省西安市710032

出　　处：《世界华人消化杂志》2001年第5期541-545,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金 No.39270744

摘　　要：目的检测HCV基因及其抗原在人原发性肝内胆管细胞癌中的表达,探讨肝内胆管细胞癌的病因及发病机制。方法用免疫组化技术检测HCV抗原,用原位分子杂交技术检测HCV基因(HCV RNA)。结果在40例肝内胆管细胞癌中,HCVC33c抗原、核心抗原及HCV RNA阳性率分别为52.5％,72.5％和57.5％;在21例癌旁肝组织中,其阳性率分别为63.6％,45.5％和48.5％。结论人原发性肝内胆管细胞癌的发生除与HBV有密切关系外,与HCV慢性感染也有密切关系。AIM In order to explore the etiology and pathogenesis of human primary intrahepatic cholangiocarcinoma, expression of HCV genes and its antigens in cancer and its surrounding hepatic tissues was detected. METHODS HCV antigens were detected by immunohistochemical technique and HCV genes by in situ hybridization. RESULTS Among 40 cases of intrahepatic cholengiocarcinoma, the positive rate of HCV C33c, core antigen and HCV RNA was 52.5%, 72.5% and 57.5%, respectively; in 21 cases of cancerous surrounding hepatic tissues, 63.6%, 45.5% and 48.5%, respectively. CONCLUSION In the pathogenesis of the intrahepatic cholangiocarcinoma, there exists a close relationship between human primary intrahepatic cholangiocarcinoma and besides HBV, and HCV chronic persistent infection.

关 键 词：原发性肝内胆管细胞癌 HCV基因 HCV抗原 发病机理 

分 类 号：R735.8[医药卫生—肿瘤]