单克隆抗体检测大便样品诊断大肠癌的研究  

Diagnosis of colorectal cancer by using anti-colorectal cancer monoclonal antibody

作　　者：李力军[1] 杜伟生[1] 冯常智[2] 张世俊[2] 牛刚[3] 蒋志新[2] 高德禄[2] 谭焕然[4] 

机构地区：[1]铁道部北京铁路总医院,北京100038 [2]中国人民解放军305医院 [3]北京牛牛基因技术有限公司 [4]北京大学医学部基础医学院药理学部

出　　处：《中国肿瘤临床与康复》2001年第3期5-7,共3页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：北京铁路总医院科学研究基金! (资助号 :980 0 0 9);科学部生命科学技术发展中心2000年度项目!(资助号 :96-90 1-0 5 -197);北京市科

摘　　要：目的　应用抗人大肠癌单克隆抗体对正常人群进行筛选和大肠癌诊断的研究。方法　采用ELISA法对正常人群和病理切片确诊为大肠癌的患者以及大肠癌患者手术前后的大便样品进行对比研究。结果　应用本抗人大肠癌单克隆抗体 (Anti colorectalcancerantibody ,Anti Cca)可以通过检测大便进行正常人群的筛查 ;对大肠癌患者手术前后的大便进行对比性研究发现 ,手术前大便的检测结果呈强阳性 ,手术后为阴性。对临床病理检查已经确诊为大肠癌的患者 ,再采用抗人大肠癌单克隆抗体检测大便诊断大肠癌 ,两者的吻合率为 10 0 %。结论　本实验所使用的大肠癌单克隆抗体可用于从大便中检测大肠癌的脱落细胞 ,可用于正常人群的筛查和进行大肠癌的诊断研究。Objective To screen and diagnose the human colorectal cancer by using anti-colorectal cancer monoclonal antibody.Methods The colorectal cancer antigen was detected by using ELISA method in stool samples collected from normal controls and pathologically proved patients before and after operation.Results Anti-colorectal cancer monoclonal antibody could be applied to screening colorectal cancer in normal controls by using ELISA method.In the comparative study of patients with colorectal cancer,pre-operative stool samples showed a positive result,and it turned to negative after operation.The detectability of the colorectal cancer antigen in stool samples tallied with that of pathological examination.Conclusions Anti-colorectal cancer antibody can be used to detect the colorectal cancer antigen, to screen and diagnose in normal people.

关 键 词：大肠癌 抗大肠癌单克隆抗体 诊断 

分 类 号：R735.3[医药卫生—肿瘤]