带有白花甜菜染色体的栽培甜菜单体附加系RAPD分析适宜引物的筛选  

Screening of optimal primers of RAPD analysis in monosomic addition lines of B. Vulgaris L. with a chromosome of B. corolliflora Zoss

作　　者：康传红[1] 韩晓云[1] 郭德栋[1] 王桂芝[1] 

机构地区：[1]黑龙江大学生物工程系,黑龙江哈尔滨150080

出　　处：《黑龙江大学自然科学学报》2001年第3期108-110,共3页Journal of Natural Science of Heilongjiang University

基　　金：黑龙江省科委青年基金资助项目(QOOOO18)

摘　　要：运用 ６８个 １０ｂｐ随机引物对白花甜菜、载培甜菜、带有白花甜菜染色体的栽培甜菜单体附加系进行了ＰＣＲ扩增，所选引物中有２个扩增产物不明显，４４个引物扩增产物在所选材料中无多态性，２２个引物的扩增产物对白花甜菜具有特异特殊异片段，这 ２２个引物将可以用于白花甜菜单体附加系的ＲＡＰＤ分析研究。We have performed the RAIPD analysis in B.corolliflora Zoss., B.Vulgaris L., monosomic addition lines of B.corolliflora. Of total tested 68 random primers, the amplification products of two primers are not visible; 44 primers have consistent amplification products in selected materials; 22 amplification products have specific fragment in B. corolliflora. Thes 22 random primers will be applied to RAPD analysis of B. Vulgaris monosomic addition lines.

关 键 词：白花甜菜 栽培甜菜 单体附加系 RAPD 引物 筛选 

分 类 号：S566.3[农业科学—作物学]