高温诱导双孢蘑菇蛋白质表达变化的分析  

Analysis of the Protein Expression of Agaricus bisporus Strains after Growing at High Temperature

作　　者：白雪[1] 陈兰芬[1] 宋思扬[1] 黄耀坚[1] 苏文金[1] 陈美元[2] 王泽生[2] 

机构地区：[1]厦门大学生命科学学院 [2]福建省轻工业研究所,福建福州350005

出　　处：《厦门大学学报（自然科学版）》2001年第6期1342-1345,共4页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金资助 (C982 0 0 0 5 )

摘　　要：对 2 4℃常温培养和 32℃诱导培养的双孢蘑菇 82 13、0 2、2 796、4 60 7菌株的菌丝体总蛋白质的 SDS- PAGE分析比较 ,发现 2 796和 4 60 7菌株在 4 3k D～ 110 k D之间有明显的差异蛋白质出现 ,而 0 2和 82 13菌株的蛋白质差异带不明显 .这些高温诱导特异表达的蛋白质可能与双孢蘑菇的热耐受相关 .Agaricus bisporus strains (02821327964607) from a genetic family were cultured at different temperature (32 ℃ and 24 ℃). Analysis of the protein pattern with SDS-PAGE of those strains showed that strain 2796 and 4607 have many induced protein bands between 43 kD～110 kD, but strain 02 and strain 8213 have few induced protein bands. Those results suggested that the thermotolerance character is related to the different genomic construct of different strains.

关 键 词：双孢蘑菇 高温诱导 蛋白质差异表达 食用菌 菌丝生长 液体培养 

分 类 号：S646.11[农业科学—蔬菜学] Q935[农业科学—园艺学]