肝纤维化大鼠肝窦内皮细胞整合素α_6β_1及粘着斑激酶表达的变化  

Changes of the expression of integrin α_６β_１ and the activity of focal adhesion kinase in the liver sinusoidal endothelial cell in experimental fibrotic rats

作　　者：刘小菁[1] 傅华[2] 杨丽[3] 黄明慧[1] 肖文君[3] 王一平[3] 

机构地区：[1]华西医科大学附属第一医院内科实验室,成都610041 [2]华西医科大学附属第一医院心内科,成都610041 [3]华西医科大学附属第一医院消化内科,成都610041

出　　处：《中华肝脏病杂志》2001年第6期349-351,共3页Chinese Journal of Hepatology

基　　金：国家自然科学基金(39700068)

摘　　要：目的 研究正常及肝纤维化时大鼠肝窦内皮细胞（ＳＥＣ）表面层粘连蛋白（ＬＮ）的整台素受体α６β１及粘着斑激酶（ＦＡＫ）的变化。方法 用胶原酶原位灌注、Ｐｅｒｃｏｌｌ不连续密度梯度离心法分离正常及四氯化碳（ＣＣｌ４）实验性大鼠肝纤维化模型中的ＳＥＣ，并进行体外培养。采用细胞－ＥＬＩＳＡ和免疫沉淀－蛋白质酪氨酸激酶活性测定的方法，分别观察ＳＥＣ细胞表面整合素α６β１表达及ＦＡＫ活性的变化。结果 正常大鼠ＳＥＣ细胞表面几乎不表达整合素α６β１；在肝纤维化时ＳＥＣ细胞表面α６β１蛋白表达却明显增加（Ｐ＜０．０５），且细胞中ＦＡＫ活性明显增高（Ｐ＜０．０５）。结论 在肝纤维化时ＳＥＣ细胞表面整合素α６β１的表达及ＦＡＫ活性明显增高，可能在ＳＥＣ的形态及功能改变中起重要作用。Objective To investigate the expression of integrin α６β１ and the activity of focal adhesion kinase（FAK) in liver sinusoidal endothelial cells（SECs) from experimental fibrotic rats induced by CCl4. Methods By in situ collagenase perfusion and two-step Percoll gradient centrifugation, SECs were isolated and cultured from normal and CCl4-treated Wistar rats. The expression of integrin α６β１ was determined by cell-ELISA, and the activity of FAK was assessed by immunoprecipitation-tyrosine kinase assay. Results The integrin α６β１ was almost absent in the normal SECs and was up-regulated during the fibrotic process; SECs from experimental fibrotic rats possessed higher expression level of integrin α６β１ than normal SECs（P<0.05). The FAK activity in SECs from experimental fibrotic rats increased significantly as compared with the normal controls（P<0.05). Conclusions The expression of integrin α６β１ on SECs and the increase of FAK in SECs may be important in the phenotype and function changes of SECs during hepatic fibrogenesis.

关 键 词：肝纤维化 肝窦内皮细胞 整合素 粘着斑激酶 大鼠 

分 类 号：R575.2[医药卫生—消化系统]