在大肠杆菌中表达蜘蛛拖丝融合蛋白  

Production of GST Fused Spider Dragline Silk Fiber Protein ADF3 in E.coli

作　　者：牛恒尧[1] 王义琴[1] 萨其拉[1] 李文彬[1] 孙勇如[1] 

机构地区：[1]中国科学院遗传研究所,北京100101

出　　处：《高技术通讯》2001年第11期5-7,共3页Chinese High Technology Letters

摘　　要：蜘蛛丝凭借其优良的理化性质倍受人们青睐 ,但是蜘蛛本身难以高密度养殖给应用造成巨大的障碍。由此利用基因工程的手段来开发利用蜘蛛拖丝蛋白目前成为各国研究的热点。以往的研究表明 ,不同的编码蛛丝蛋白的cDNA片段在表达中的稳定性各不相同。在应用中存在适宜片段的筛选问题。本文从该角度出发 ,首次建立了从Ara neusdiadematu克隆的拖丝蛋白基因ADF3与GST融合产物的稳定原核表达系统。为今后进一步开发利用蜘蛛拖丝蛋白的研究奠定了基础。Spider silk fiber has long been regarded as powerful potential new material for its high strength, tension and energy need for break. Anyway, because of their cannibalism, It is almost impossible for a high-density culture. This limits the further application of spider silk fiber protein severely. So, nowadays, most of our attention is on possible production of spider silk fiber protein through genetic engineering. As previous research indicated differential spider silk fiber cDNA segments showed various stability when cloned into a prokaryotic expression system. Then, suitable cDNA becomes a key point when consider applying spider silk fiber gene for genetic engineering. Here, we present the first report on stable expression of GST fused Araneus diadematu dragline silk fiber protein ADF3 in E.coli, which in turn shows that ADF3 maybe a promising target cDNA in future applications.

关 键 词：蜘蛛丝 Araneusdiadematu 原核表达 基因工程 大肠杆菌 丝类纤维 蜘蛛拖丝融合蛋白 

分 类 号：Q591.2[生物学—生物化学]