蛇毒制剂对兔眼滤过术的影响  

Experimental research of inhibition of scar formation from glaucoma filtration surgery by viper venom drug

作　　者：刘金华[1] 魏厚仁[2] 吕源淑[2] 张明昌[2] 

机构地区：[1]广西医科大学第一附属医院眼科,南宁530020 [2]同济医科大学协和医院眼科

出　　处：《眼科研究》2001年第5期436-438,共3页Chinese Ophthalmic Research

基　　金：广西壮族自治区教育厅基金资助项目(编号:99001)

摘　　要：评价蛇毒制剂在兔眼滤过术后抗瘢痕形成的效果和眼毒性。方法 对１０只兔双眼行巩膜全层咬切术，术后实验组球结膜下注射 ５ × １０－２Ｕ／ｍｌ的蛇毒制剂０．５ ｍｌ，对照组注射等量生理盐水，裂隙灯观察眼前段。术后第２１天，用鼠尾胶原作为房水外流示踪剂行前房恒压灌注及原位固定，对手术区组织行功能及组织形态学检查。结果术后１４，２１天，实验组有５眼、３眼可见滤过泡，对照组无１眼有滤过泡。滤过泡消失眼的滤过口被瘢痕组织封闭，手术区结膜下纤维组织明显增生。有滤过泡眼的滤过口仍显开放，房水示踪剂经此开口流入滤过泡。结论 蛇毒制剂能抑制兔眼滤过术后结膜下过分瘢痕化，延长滤过泡的存在时间和功能，其眼毒性不明显。Objective To evaluate the inhibiting effect of viper venom drug (Ahalysantipfarciasi) on scar formation following glaucoma filtration surgery and it's ocular toxicities. Methods Twenty eyes of 10 rabbits received sclerectomy. Viper venom drug(5 × l0 -2 U/ml,0. 5 ml) was given to one eye every other day for 3 times(the experimental group). The same volume of saline solution was applied to the opposite eye(the control group) in the same rabbit. On day 21,the rat-tail collagen as a tracer of the aqueous humor outflow was perfused into the anterior chamber of both groups at equal, steady IOP pressure. The eyes were subjected to fixation in situ and the morphology of operated tissue was observed by light microscope. Results The filtering blebs of 5,3 eyes survived on day 14 and 21respectively in Exp. group,but none in the control group. The eyes without bleb had closure of the sclerostomy with scar tissue and TFs proliferation in episclera. The aqueous humor tracer can be found outflowing into the bleb in ones with bleb. Conclusions 5 × l0 -2 U/ml of drug can inhibit scar formation following glaucoma filtering surgery. Its ocular toxcities was not evident.

关 键 词：蛇毒制剂 青光眼滤过术 抗瘢痕形成 眼毒性 

分 类 号：R779.6[医药卫生—眼科]