^(125)IUdR对人脑胶质瘤细胞SHG44的杀伤作用  

Experimental study of the killing effect on human brain glioma SHG44 cells by ^(125)IUdR

作　　者：李金泉[1] 鲍耀东[1] 周岱[1] 吴翼伟[1] 江一民[1] 王博诚[2] 金坚[2] 

机构地区：[1]苏州大学附属第一医院,苏州215006 [2]核医学国家重点实验室,无锡214063

出　　处：《核技术》2001年第11期881-884,共4页Nuclear Techniques

基　　金：核医学国家重点实验室开放科研基金资助

摘　　要：为评价12 5IUdR对人脑胶质瘤细胞SHG4 4的杀伤作用 ,把12 5IUdR加入到SHG4 4细胞的培养基中 ,孵育后测量细胞摄取12 5IUdR的放射性活度 ;采用克隆形成法测定12 5IUdR对SHG4 4细胞生长抑制的效果。结果表明 ,培养基中12 5IUdR浓度增加时 ,SHG4 4细胞对12 5IUdR的摄取量也相应增加 (相关系数r =0 .9917)。SHG4 4细胞摄取12 5IUdR后生长受到明显抑制 ,细胞存活分数与培养基中12 5IUdR浓度呈直线负相关 (r =- 0 .9736 ) ,其半数致死剂量LD50 为 (8.7± 0 .12 )kBq/mL ,12 5IUdR组的SHG4 4细胞存活分数明显低于Na12 5I组 (P <0 .0 0 1)。结果提示 ,12 5IUdR能够掺入到SHG4 4细胞中 ,12 5IUdR对SHG4 4细胞有明显的杀伤作用 ,说明12 5IUdR可望成为治疗脑胶质瘤的潜在的一种放射性药物。To evaluate the cytocidal effect of 125 IUdR in human glioma cell line SHG44, 125 IUdR were added to the RPMI1640 culturing medium. The amount of 125 IUdR uptake by SHG44 cells was evaluated through measuring the radioactivity per cell; the killing effects of 125 IUdR on SHG44 cells were estimated by colony forming method. The amounts of 125 IUdR uptake by SHG44 were growing up as a function of 125 IUdR dose in the medium ( r =0.9917). The surviving fraction was correlated with the concentration of 125 IUdR ( r =-0.9736). The LD 50 of 125 IUdR group was (8.7±0.12)kBq/mL and the surviving fraction of 125 IUdR group was significantly lower than that of Na 125 I group ( P <0.001). 125 IUdR can be incorporated into the SHG44 cells, and the concentration of 125 IUdR in SHG44 cells was influenced by 125 IUdR dose in the medium. The inhibitive effects of 125 IUdR on SHG44 cells were obviously.

关 键 词：脑胶质瘤 脱氧尿嘧啶核苷 SHG44细胞 放射性核素 治疗 碘125 放射疗法 放射性药物 杀伤作用 标记物 

分 类 号：R739.41[医药卫生—肿瘤] R730.55[医药卫生—临床医学]