彗星分析法检测γ射线诱导的人肝癌细胞DNA链断裂  

DNA strand breakage of human hepatoma cells induced by γ-ray measured using the Comet Assay

作　　者：王菊芳[1] 何静[1] 周光明[1] 李文建[1] 卫增泉 高清祥[2] 

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]兰州大学,兰州730000

出　　处：《核技术》2001年第11期895-898,共4页Nuclear Techniques

基　　金：中国科学院"西部之光"资助项目 (XB980 6 0 4)

摘　　要：以人肝癌SMMC - 772 1细胞为材料 ,用彗星分析法研究了γ射线诱导的DNA链断裂 ,结果表明 ,随着剂量的增加 ,彗尾逐渐变长 ,彗尾的细胞数也越来越多 ;彗尾与彗头的长度比和面积比与剂量成线性正相关 ,尤以长度比随剂量的增加变化为明显 ,可反映γ射线诱导的DNA链断裂程度。In this paper, DNA strand breakage induced by γ-ray was studied using the Comet Assay. The human hepatoma cells were chosen as the test cell line. The results showed that the length of the tail appeared longer as the dose increased, and the number of cells with tail, increased with increasing dose. The length ratio and area ratio had positive correlation with the dose. Among them, the length ratio showed more obvious change with the increasing dose than area ratio, thus it could be used to describe the level of DNA strand breakage simply and efficiently.

关 键 词：慧星分析 Γ射线 人肝癌细胞 DNA 链断裂 DNA链 断裂 细胞凋亡 Γ射线 电离辐射 辐射损伤 

分 类 号：R735.7[医药卫生—肿瘤] Q691[医药卫生—临床医学]