丙泊酚、咪达唑仑和硫喷妥钠对鼠局灶脑缺血部位氨基酸堆积的影响(英文)  

Effects of propofol, midazolam and thiopental sodium on amino acid accumulation in focal cerebral ischemic areas in rats

作　　者：陈莲华[1] 贡沁燕[2] 高慧[3] 陈英子[1] 肖常思[1] 

机构地区：[1]复旦大学附属眼耳鼻喉科医院麻醉科,上海200031 [2]复旦大学基础医学院药理教研室,上海200032 [3]复旦大学基础医学院神经生物实验室,上海200032

出　　处：《中国新药与临床杂志》2001年第6期401-405,共5页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的 :观察丙泊酚、咪达唑仑和硫喷妥钠对鼠局灶脑缺血再灌注后缺血部位氨基酸堆积的影响。方法 :雄性SD大鼠 ,大脑中动脉内线栓法诱发3h局灶脑缺血和 3h再灌注。于再灌注前分别给予丙泊酚、咪达唑仑和硫喷妥钠 5 0mg·kg- 1,ip。再灌注 3h后用高效液相色谱法 (HPLC)测脑组织氨基酸浓度。结果 :丙泊酚和咪达唑仑减少缺血组织内门冬氨酸和谷氨酸的堆积。丙泊酚增强 ,咪达唑仑减小γ -氨基丁酸的堆积。硫喷妥钠无明显作用。结论 :丙泊酚和咪达唑仑可能通过加快再灌注时兴奋性氨基酸的清除而产生脑保护效应。AIM: To investigate the effects of propofol, midazolam and thiopental sodium on amino acid accumulation in ischemic areas in rats subjected to focal cerebral ischemia reperfusion. METHODS: Male SD rats were scheduled to undergo 3 h middle cerebral artery occlusion by intraluminal suture and 3 h reperfusion. The treated rats were given propofol, midazolam and thiopentol sodium 50 mg·kg -1 ,ip respectively at the beginning of reperfusion. After 3 h reperfusion concentrations of various amino acids (aspartate, glutamate, glycine, taurine, and GABA) were measured with high performance liquid chromatography (HPLC) system. RESULTS: Propofol and midazolam attenuated aspartate and glutamate accumulation in ischemic areas. Propofol augmented, but midazolam diminished, GABA accumulation. Thiopental sodium had no effect on the dynamics of amino acids. CONCLUSION: Propofol and midazolam, but not thiopental sodium, may provide neuroprotection through the acceleration of excitatory amino acid elimination during reperfusion.

关 键 词：丙泊酚 咪达唑仑 硫喷妥钠 氨基酸 脑缺血 再灌注 

分 类 号：R971.2[医药卫生—药品] R743.31[医药卫生—药学]