HPLC测定葛根芩连汤不同煎液中葛根素的含量  

DETERMINATION OF THE CONTENT OF PUERARIN IN DIFFERENT DECOCTIONS OF PUERARIAE DECOCTION BY HPLC

作　　者：梁光义[1] 曹佩雪[2] 徐必学[1] 陈丹[1] 李霞 

机构地区：[1]贵州,中国科学院天然产物化学重点实验室,贵州贵阳550002 [2]贵阳中医学院,贵州贵阳550002 [3]贵州宏宇药业有限公司,贵州贵阳550002

出　　处：《华西药学杂志》2001年第5期371-373,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助课题 (No :3996 0 0 83)

摘　　要：目的 :考察用合煎、分煎和沸水冲泡单味中药精制颗粒等方法制备的葛根芩连汤汤剂中葛根素含量的变化。方法 :采用HypersilC18柱 (5 μ ,id 4. 6mm× 2 5 0mm) ,流动相甲醇 水 (30∶70 ) ,流速 1ml·min 1,柱温2 5℃ ,检测波长 2 5 0nm ,外标法定量。结果 :葛根芩连汤分煎液中葛根素的平均含量为 2 8. 74mg ,合煎液中平均含量为 2 3. 0 1mg,单味中药精制颗粒冲泡液中平均含量为 13. 37mg。结论OBJECTIVE:To determine the puerarin in different decoctions of puerariae decoction METHODS:The HPLC was used for the determination. Chromatographic conditions: Hypersil C 18 column(5μ,ID 4.6 mm×250 mm), methanol water (30∶70) as the mobile phase, the wavelength was 250 nm.RESULTS:The average content of puerarin was 23.01 mg in hejian decoction (preparing the decoction as traditional method,that is,mixed the traditional Chinese herbs together, then boiling them with water), 28.74 mg in fenjian decoction (boiling the single traditional Chinese herb with water separately, then mixed the solutions)and 13.37 mg in decoction of refined granules (preparing the refined granules of single traditional Chinese herb from different kinds of traditional Chinese herbs separately then mixing the granules with boiling water).CONCLUSION:The content of puerarin in fenjian decoction is the highest, that in the decoction of refined granules is the lowest.

关 键 词：葛根芩连汤 葛根素 合煎 分煎 中药精制颗粒 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]