玉米自交系幼胚培养与基因枪转化研究  

Study on Maize Inbred Immature Embryo Culture and Transformation by Particle Bombardments

作　　者：蒋武生[1] 郅玉宝[1] 耿建峰[1] 原玉香[1] 张晓伟[1] 韩永平[1] 陈占宽[1] 高睦枪[1] 

机构地区：[1]河南省农业科学院生物技术研究所,郑州450002

出　　处：《河南农业科学》2001年第10期4-6,共3页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金资助项目 (3970 0 0 91)

摘　　要：玉米自交系幼胚在添加 1～ 2mg/L 2 ,4-D的N6培养基上能够诱导形成不同类型的胚性愈伤组织。愈伤组织的诱导率与基因型和 2 ,4-D浓度有关 ,6个自交系的诱导率在 5 2 %～ 89%。将淡黄色颗粒状结构致密型愈伤组织在N6诱导培养基上继代培养 2 0个月 ,仍具有较强的分化能力。在附加 2mg/L 6 -BA和0 .2mg/LNAA的N6分化培养基上 ,有 2 7%～ 41%的愈伤组织分化成苗。通过基因枪轰击成功地将 pbarGUS基因转入玉米细胞 ,获得了大量的转化胚性愈伤组织。用 0 .4mol/L的甘露醇高渗处理幼胚 ,能显著提高pbarGUS基因在细胞中的表达 ,高渗处理转化率较对照提高了Study on factors in fluencing maize immature embryo culture and pbar GUS gene transformation by particle bombardments have been studied.Showed that genetypes and 2,4-D had some effect on embryonic callus induction.Adding 2 mg/L 2,4-D to N\-6 medium was better in callus induction than adding 1 mg/L 2,4-D.The callus induction of 52%～89% had been obtained in 6 inbreds.A lot of embryonic callus could got by introducing pbar GUS gene into maize cell with particle bombardment.High osmotic treatment on immature embryo with 0.4mol/L mannitol could improve transient expression of pbar GUS gene,with the transformation rate increasing 3 times than ck.

关 键 词：玉米 自交系 幼胚培养 愈伤组织 pbarGUS基因 基因枪 基因转化 

分 类 号：S513.03[农业科学—作物学]