电化学疗法与恶性淋巴瘤细胞凋亡及相关基因表达  

ECT and apoptosis and apoptosis-related gene expression in malignant lymphoma

作　　者：张文霞[1] 孙慧[2] 王立新[3] 多耀祥[1] 李莉[1] 王志光[1] 

机构地区：[1]天津医科大学生理教研室,300070 [2]天津肿瘤医院 [3]天津市第一医院

出　　处：《天津医科大学学报》2001年第3期308-309,共2页Journal of Tianjin Medical University

基　　金：天津市自然科学基金课题 (编号 :993608211)

摘　　要：目的 :探讨电化学疗法(ECT)与肿瘤细胞凋亡的关系。方法 :采用Tunel法和免疫组化S -P法 ,分别检测肿瘤未治疗对照组和ECT组B细胞淋巴瘤组织的细胞凋亡率和p53、bcl-2基因的表达。结果 :ECT组肿瘤细胞凋亡率(5.18)显著高于对照组(1.05) ,p53、bcl-2在ECT组表达分别为80%、25 % ,在对照组表达分别为15 %、90%。ECT组中p53表达与癌细胞凋亡率呈正相关 ,而bcl-2则与癌细胞凋亡率呈负相关。结论 :ECT可诱导癌细胞凋亡 。Objective: To study the relationship of ECT and apoptosis of malignant lymphoma.Methods:Using Tunel and immunohisytochemistry S-P method, the apoptotic rate(AR) and expression of p53 and bcl-2 protein of B-lymphoma in ECT and control group were examined.Results: Few apoptotic cell were found in control group(P<0.001), the AR significantly lower than that of ECT. The frequency of expression of p53 and bcl-2 in ECT was 80% and 25%, respectively, while that in control group was 15% and 90%. In ECT, expression of p53 was positively correlated, while bcl-2 negatively correlated with the AR(P<0.001).Conclusion: Apoptosis is induced in ECT which may be related to p53 and bcl-2 gene regulation.

关 键 词：电化学疗法 细胞凋亡 P53基因 BCL-2基因 恶性淋巴瘤 治疗 

分 类 号：R733.405[医药卫生—肿瘤]