重组人白介素10对离体大鼠胸主动脉血管平滑肌细胞增殖的影响  

Effect of recombinant human interleukin-10 on the in vitro proliferation of rat vascular smooth muscle cells

作　　者：欧阳平[1] 刘煜[1] 赖文岩[1] 彭立胜[2] 杨红[2] 吴文言[2] 徐安龙[2] 

机构地区：[1]第一军医大学南方医院心内科,广东广州510515 [2]中山大学生命科学学院,广东广州510275

出　　处：《第一军医大学学报》2001年第11期828-830,共3页Journal of First Military Medical University

基　　金：广州市科委科技攻关项目(JB00000448165)

摘　　要：目的 观察重组人白介素１０（ｒｈＩＬ－１０）对血管平滑肌细胞（ＶＳＭＣ）增殖的影响。方法 离体培养大鼠胸主动脉 ＶＳＭＣ并分别接受不同浓度ｒｈＩＬ－１０、肿瘤坏死因子（ＴＮＦ－α）、血小板源性生长因子（ＰＤＧＦ－ＢＢ）的刺激，采用ＭＴＳ／ＰＥＳ 法确定ＶＳＭＣ的增殖状态并与对照组比较。结果　与对照组相比，ＴＮＦ－α和ＰＤＧＦ－ＢＢ均对ＶＳＭＣ增殖具有明显的刺 激作用（Ｐ＜０．０５）。单独应用ｒｈＩＬ－１０对血管平滑肌细胞生长没有影响。在ＴＮＦ－α和ＰＤＧＦ－ＢＢ分别刺激下，ｒｈＩＬ－１０均可 抑制ＶＳＭＣ的生长（Ｐ<０．０５）。结论　ｒｈＩＬ－１０可抑制细胞因子和生长因子诱导的ＶＳＭＣ增殖。Objective To observe the effects of recombinant human interleukin-10 (rhIL-10) on the proliferation of rat vascular smooth muscle cells (VSMCs) cultured in vitro. Method Aortic VSMCs cultured in vitro were treated with varied doses of rhlI-10 alone or in combination with tumor necrosis factor-a (TGF-a) or platelet-derived growth factor-BB(PDGF-BB) respectively. The VSMC proliferation was quantified by colormetric assay. Results Compared with control, both TNF-a and PDGF-BB stimulated conspicuous proliferation of VSMCS, bit the effect of which was not observed by rhIL-10 treatment alone. In the presence of rhlL-10, the proliferation effects of both TGF-a and PDGF-BB on VSMCs were significantly inhibited (P<0.05). Conclusion rhIL-10 can inhibit VSMC proliferation induced by TNF-a and PDGF-BB, thus may provide a new therapeutic approach for regulating vascular wall remodeling after vascular injury.

关 键 词：血管平滑细胞 重组白细胞介素-10 胸主动脉 肿瘤坏死因子 VSMC 细胞增殖 RHIL-10 

分 类 号：R543.5[医药卫生—心血管疾病]