蓝藻光合基因psaC在酵母中的转化和表达  

Expression of Photosynthetic Gene psaC of Cyanobacteria in Saccharomyces cerevisiae

作　　者：李斌[1] 李涛[1] 童雪梅[1] 王东辉[1] 赵进东[1] 吴光耀[1] 

机构地区：[1]北京大学生命科学学院,北京100871

出　　处：《北京大学学报（自然科学版）》2001年第6期754-759,共6页Acta Scientiarum Naturalium Universitatis Pekinensis

摘　　要：聚球蓝藻 (Synechococcussp .PCC 70 0 2 )的psaC是光系统I的FA FB 脱辅基蛋白基因 ,其分子量 8 9kD蛋白中含有 2个 4Fe 4S中心。构建了psaC基因的表达载体pYES2 psaC ,将表达载体转化到酵母 (Saccharomycescerevisiae)中 ,Southern杂交证明psaC基因已被转化 ,诱导培养后的蛋白电泳显示psaC基因获得高效表达。The psaC Gene of Synechococcus sp. PCC 7002 encodes the F A/F B aprotein of photosystem I,a protein with a molecular weight of 8.9 kD containing two 4Fe-4S centers.The expression vector of psaC gene was constructed,and transformed into Saccharoromyces cerevisiae.Southern blotting confirmed that psaC gene had been successfully transformed and Tris-Tricine-PAGE revealed that psaC gene had been highly expressed after incubating the transformed strains in the inducing culture.

关 键 词：聚球蓝藻 PSAC 酵母 基因转化 光合基因 基因表达 表达载体 psaC蛋白 含铁量 

分 类 号：Q78[生物学—分子生物学] Q949.22