南海红树林真菌(1356号)多糖分离提取及甲醇解研究  

Study on the Methanolysis of the Fungal Polysaccharide from the Marine Mangrove Fungus(Strain No.1356) from the South China Sea

作　　者：佘志刚[1] 胡谷平[1] 吴耀文[1] 林永成[1] 吴雄宇[1] 

机构地区：[1]中山大学化学与化学工程学院,广东广州510275

出　　处：《中山大学学报（自然科学版）》2001年第6期123-124,共2页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目 (2 96 72 0 5 3;2 0 0 72 0 5 8);广东省自然科学基金资助项目 (980 371)

摘　　要：从南海海洋真菌 (135 6号 )菌体中分离到一种新的杂多糖W11,甲醇解研究表明W11是由葡萄糖和半乳糖组成的 ,其摩尔比为 3∶2 .药理研究表明 ,该多糖能提高机体的免疫功能 ,对人肝癌细胞HepG2和Bel740 2有细胞毒作用 ,半数杀伤浓度 (IC50 )分别为 5 0 μg/mL和 2 5A fungal polysaccharide W 11 was isolated and purified from the marine mangrove fungus (strain No.1356) from the South China Sea. The methanolysis of W 11 was studied. The reaction products were trimethylsilylated and analyzed quantitatively by GC/MS.The results indicated that W 11 consists of glucoses and galactoses. Their mole rate is 3∶2.The polysaccharide showed cytotoxic effect to human hepato cellular cancer cells,HepG2 and Bel7402.The IC50 equals 50 μg/mL and 25 μg/mL respectively.

关 键 词：真菌多糖 甲醇解 三甲硅醚化反应 多糖类药生物 免疫功能 分离 提取 

分 类 号：Q936[生物学—微生物学] R977.6[医药卫生—药品]