鸡传染性支气管炎病毒嗜腺胃毒株(ZJ971)株S2基因的克隆及序列分析  

Cloning and Sequencing S2 Gene of Avian Proventriculopathic Infectious Bronchitis Virus Chinese Isolate ZJ971

作　　者：沈行燕[1] 周继勇[1] 丁红梅[1] 吴建祥[1] 严庆丰[1] 

机构地区：[1]浙江大学动物预防医学研究所,浙江杭州310029

出　　处：《中国兽医学报》2001年第6期540-542,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目 ( 39970 0 30 );浙江省自然科学基金资助项目 ( 399411);浙江省重点科研项目( 99110 2 0 30 )

摘　　要：采用特异性引物对鸡传染性支气管炎病毒 (IBV )嗜腺胃毒株 (ZJ971毒株 )进行 RT- PCR扩增 ,将扩增到的 S2基因 c DNA克隆入 p Bluescript SK质粒载体中 ,经鉴定后测序。结果显示 ,扩增的 ZJ971毒株 S2基因全长为 1872 nt,编码 1条由 5 30个氨基酸组成的多肽 ,与 Beaudette株、M41株、Ark99株比较 ,其核苷酸同源性分别为 97.34 %、97.72 %、96 .30 % ;3′端核苷酸相对于 5′端保守 ,在 5′端第 184～ 2 83位之间的核苷酸为高可变区。同时 ,IBV - ZJ971株S2基因第 16 2 1位的碱基由 G突变为 T,导致The cDNA of S2 gene of avian proventriculopathic infectious bronchitis virus(IBV) Chinese isolate ZJ971 was amplified by RT-PCR and then cloned into vector pBluescript SK.The resulting plasmid was identified by restriction enzyme for the S2 insert which was eventually sequenced.The sequencing data indicated that the S2 gene cloned comprised an 1 872 nucleotides sequence encoding a 530 amino acids polypeptide.The sequence comparison of the S2 gene with the international strains M41,Ark99 and Beaudette showed that the homolgy of IBV ZJ971 was 97.34%,97.72% and 96.30% respectively.It was also evidenced that the 3′ region of ZJ971 S2 gene was more conservative than its 5′ region where located a highly variable sequence between nucleotide 184-283.In additon,a G to T site mutation was first found at nucleotide 1 621 which lead a emerging of a stop code TAA,presumably resulting in translation termination of S2.

关 键 词：鸡 传染性支气管炎病毒 S2基因 基因克隆 序列分析 

分 类 号：S858.31[农业科学—临床兽医学] S852.65[农业科学—兽医学]