姜黄素在急性髓性白血病HL-60细胞中对凋亡调控蛋白Bax、Bak、Mcl-1的影响  

The Study of the Effect of Curcumin on Apoptosis-Regulating Protein Mcl-1, Bax and Bak in Acute Myeloid Leukemia HL-60 Cell Line

作　　者：吴裕丹[1] 陈燕[1] 何静[1] 陈文娟[1] 李慧玉[1] 向建平[1] 董继华[1] 何明生[1] 向直富[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液病研究所,武汉430022

出　　处：《同济医科大学学报》2001年第1期25-27,共3页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金资助项目 (No.39770 934 )

摘　　要：为了探讨姜黄素在诱导急性髓性白血病 HL- 6 0细胞凋亡时 ,Bcl- 2基因家族成员是否参与了其调控过程。选用 Bcl- 2基因家族成员 Mcl- 1、Bax、Bak蛋白为研究对象 ,SABC法检测其表达状况 ;用流式细胞术测定 DNA直方图中 Sub- G1 峰值、末端 Td T酶标技术检测 TUNEL 细胞阳性率。发现当 2 5 μmol/ L 姜黄素分别处理 HL- 6 0细胞 2 4h、 36h、 48h后 ,可使 Mcl- 1表达下调 ,Bax和 Bak上调 ,呈时间依赖性。在各处理组 ,Mcl- 1、 Bax、 Bak表达同对照组相比有显著差异 (F值分别为 3.44 3、 8.32 8;P值分别为 0 .0 40、 0 .0 0 1)。结果表明 :Bcl- 2基因家族成员确实参与了姜黄素诱导 HL- 6Whether or not the Bcl 2 gene family was involved in modulating mechanism of apoptosis induced by curcumin in acute myeloid leukemia HL 60 cell line was investigated. The Bcl 2 family members Mcl 1, Bax and Bak were selected as subjects. The expression of these proteins was detected by using SABC method. The attitude of Sub G1 peak in DNA histogram was determined by FCM. The TUNEL positive cell percentage was identified by terminal deoxynucleotidyl transferase (TdT) mediated Biotin dUNP end labeling technique. It was found that after treatment of HL 60 cells with 25 μmol/L curcumin for 24 h, 36 h, 48 h, respectively, the expression level of Mcl 1 was down regulated and that of Bax up regulated in a time dependent manner. In different treated groups, the expression levels of Mcl 1, Bax and Bak were significantly different from that of control group ( F values were 3 443 and 8 328, respectively and P values were 0 040 and 0 001 respectively ). It was suggested that the Bcl 2 gene family indeed participated in the regulatory process of apoptosis induced by curcumin in HL 60 cells.

关 键 词：BCL-2基因家族 姜黄素 HL-60细胞系 急笥髓性白血病 细胞凋亡 Bax BAK MCL-1 

分 类 号：R733.71[医药卫生—肿瘤]