维甲酸诱导HL-60细胞分化与细胞周期的关系  

Alteration of the Cell Cycle during the Differentiation of HL-60 Cells by Induction of Retinoic Acid

作　　者：梁英民[1] 蒋珊珊[1] 吴绒丽[1] 刘利[1] 郝淼旺[1] 邓中荣[1] 王智[1] 

机构地区：[1]第四军医大学唐都医院血液科,西安710038

出　　处：《中国实验血液学杂志》2001年第3期220-222,共3页Journal of Experimental Hematology

摘　　要：研究全反式维甲酸 (RA)在体外诱导人髓系白血病细胞系 (HL 60 )分化过程中细胞周期的改变。利用流式细胞术动态测定RA在体外诱导HL 60细胞分化过程中细胞周期的改变。结果发现 :①在RA与HL 60细胞共培养 4 8小时内 ,S +G2 /M期比例无明显变化 ,但S期细胞的比例改变与RA的浓度有关 ,在 10 - 6 mol/LRA浓度S期细胞比例先出现一过性升高 ,继而持续下降 ,在 10 - 5mol/L浓度S期细胞比例表现为持续性下降 ;②重培养试验证明 :S +G2 /M和S期细胞比例的下降与成熟分化细胞的增加相一致 ;③RA诱导HL 60细胞成熟分化并不是在一个时间点上同时进入分化状态 ,而一旦细胞开始进入成熟分化 ,即使在无RA存在的情况下 ,细胞仍能分化至成熟。结论提示 :RA与HL 60细胞必须相互作用一定时间后才能触发细胞成熟分化 ,S期细胞的比例改变与RA药物浓度有关 ,一旦细胞开始进入成熟分化 ,即使在无RA存在的情况下 。To study the alteration of the cell cycle during the differentiation of human myeloid leukemia cell line HL-60 induced with all trans-retinoic acid(RA), the flow cytometry was used to assay the various phases of cell cycle in HL-60 cells treated with RA. The results showed: ① S+G 2/M phase proportion kept relative invariability during the 48 hours incubation of HL-60 cells with RA, however, the proportion alteration of S-phase cells was associated with the RA concentrations. At 10 -6 mol/L RA, the proportion of S-phase cells appeared a temporarily increasing peak followed by persistent decrease of S-phase proportion. At 10 -5 mol/L RA, S-phase cell proportion only appeared the persistent decreasing tendency. ② Re-culture of HL-60 cells without RA showed the decrease of S+G 2/M and S-phase cells was associated with the increase of differentiated cells, but not all HL-60 cells were triggered into differentiation at the same time. Once the cells start to differentiate, even if there is no RA presence, the HL-60 cells were still differentiated until maturation. In conclusion, HL-60 cells are able to differentate and maturate after exposure to RA for a period of time. S-phase proportion is related to the concentrations of RA. Once the cells start to differentiate, even if there is no RA presence, the HL-60 cells still differentiatiated until maturation.

关 键 词：人髓系白血病细胞系 HL-60细胞系 细胞周期 细胞分化 维甲酸 

分 类 号：R733.7[医药卫生—肿瘤] R73－361[医药卫生—临床医学]