正交实验选择嗜碱细菌降解木质素的最优综合培养条件  

Optimization of Synthetic Culture Condition on Lignin Biodegradation Ability of Alkaliphilic Ligninolytic Bacteria Using Orthogonal Experimental Design

作　　者：罗宇煊[1] 张甲耀[1] 龚利萍[1] 管筱武[1] 

机构地区：[1]武汉大学化学与环境科学学院,武汉430072

出　　处：《环境科学》2001年第5期95-98,共4页Environmental Science

基　　金：国家自然科学基金资助项目 (2 96 770 12 )

摘　　要：在复合碳源的碱性液体培养条件下 ( pH≈ 1 0 5) ,用正交实验法对影响嗜碱木质素降解细菌 6号菌株降解木质素的 7种单因素培养条件进行了优化 .结果表明 ,第 1碳源用量等单因素在不同程度上对 6号菌株产生的木质素降解酶Laccase和MnP以及木质素降解率都有一定促进作用 .正交实验表明 ,其中第 1碳源用量和T 80用量是显著性的影响因素 ,7种单因素的最佳综合水平为 :37℃静置培养 1 0d、第 1碳源 (蔗糖 ) 1 g/L、氮源NH4 NO31 2 g/L、培养基 70ml/2 50ml锥形瓶、初始pH值 1 0 6和第 2碳源接种后第 4d加入、添加 0 3%的表面活性剂TThe effects of seven single culture factors on lignin biodegradation ability of alkaliphilic ligninolytic bacteria strain 6 in alkaline liquid medium (pH≈10 5) with compounded carbons were optimized using orthogonal experiments. The result showed the seven single culture factors promote the activities of Laccase and MnP and the degradation rate of wheat straw lignin in varying degrees. The synthetic result was analysised. The optimal synthetic condition was static culture (10 days culture period), 37℃, the initial pH10 6, 1g/L sugar (the starting carbons), wheat straw lignin (the second carbons, wheat straw powder 20g/L, added into on the fourth day after inoculation), 12g/L NH 4NO 3 (nitrogen), 03% detergent T80, 70ml medium/250ml flask.

关 键 词：嗜碱细菌 单因素培养条件 木质素生物降解 正交实验 最优综合培养条件 

分 类 号：X172[环境科学与工程—环境科学]