一种伤寒沙门氏菌分型的新方法  

A New Method in Typing Salmonella Typhi Strains

作　　者：底秀娟[1] 马桂芳[1] 刘彩莲[2] 

机构地区：[1]河南省卫生防疫站,郑州450003 [2]中国预防医学科学院流行病学微生物学研究所

出　　处：《中国公共卫生》2001年第7期632-633,共2页Chinese Journal of Public Health

摘　　要：目的　探讨伤寒沙门氏菌分型的新方法。方法　利用PCR扩增标记的Dig -dUTP - 16SrRNA基因探针 ,分析 40株伤寒沙门氏菌染色体经PstⅠ消化后的 16SrRNA基因限制性图谱。结果　 40株伤寒沙门氏菌的分属 14个不同的RT ,各菌株杂交片段范围为 4 4kb～ 2 9 7kb ,每个RT内有杂交带 5～ 10条不等 ,绝大多数为 5～ 6条。结论　伤寒长期在某些地区流行可能与一些核糖核酸型仅出现这些地区 ,存在相对的地区性有关 ;同一个暴发点菌株具有相同的RT。Objective To approach a new method in typing salmonella typhi strains.Method Using 16SrRNA gene,a high conservative gene in molecular evolution labeled with digoxigenin-11-dUTP by PCR,to investigate the 16SrRNA gene restriction patterns(16SrRNA GRP) of 40 straits of salmonella typhi after their chromosomes being digested with PstI.Results The PstI 16SrRNA GRP appears obviously polymorphism in strains of distinctive sources.Forty strains can be divided into 14 ribotypes.The fragments containing 16SrRNA gene are sized 4.4kb-29 7kb in 16SrRNA GRP of PstI.Most of RTs have 5-6 tapes.Conclusion The salmonella typhi's long term of prevalence maybe relate to the existance of certain types of RNA in same area.There is always the same RT in the spot of breakout.Analysis of 16SrRNA GRP can be used to trace the infections sources and transmitting routes.

关 键 词：伤寒沙门氏菌 16SrRNA 基因分型 伤寒 

分 类 号：R378.22[医药卫生—病原生物学] R516.3[医药卫生—基础医学]