Effect of Liposome-Encapsulated Total Alkaloid of Harmaline on Rabbit Lens Epithelial Cells:Experimental Study on the Prevention of Posterior Capsule Opacification  

作　　者：Mingxing Wu, Huaming Li, Shaozhen Li, Suhua PanZhongshan Ophthalmic Center, Sun Yat-sen University of Medical Science, Guangzhou 510060 , China 

出　　处：《眼科学报》1999年第1期55-60,共6页Eye Science

基　　金：This paper was supported by a grant of Public Ministry of China and Nature Science Fundation of Guangdong Province and reported in First Asian (cataract Research Conference held in Guangzhou,China 1996)

摘　　要：Purpose: To investigate whether liposome encapsulated total alkaloid of Harmaline (TAH) as a therapeutic agent is beneficial to prevention of posterior capsular opacifi-cation (PCO).Methods: Liposome-encapsulated TAH was prepared by modified freeze-thawing method. 0. 1ml of liposome-encapsulated TAH (0. 2mg/ml) was injected into the capsular bag during extracapsular lens extraction (ECLE) of each eye in total 10 rabbit eyes. Blank liposome or balance salt solution (BSS) was used as control. Slit-lamp examination and histopathological examination was used to evaluated capsule opacifica-tion. Intraocular pressure (IOP) , density and morphology of corneal endothelia cells, the amplitude and latency of b wave of ERG were measured.Results: The inflammatory response was mild both in TAH treated and the control group. PCO formation occurred in the control group 2 weeks postoperatively, but the posterior capsule was clear in TAH treated eyes. 4 weeks and 8 weeks after operation, PCO occurred both in TAHPurpose: To investigate whether liposome encapsulated total alkaloid of Harmaline (TAH) as a therapeutic agent is beneficial to prevention of posterior capsular opacifi-cation (PCO).Methods: Liposome-encapsulated TAH was prepared by modified freeze-thawing method. 0. 1ml of liposome-encapsulated TAH (0. 2mg/ml) was injected into the capsular bag during extracapsular lens extraction (ECLE) of each eye in total 10 rabbit eyes. Blank liposome or balance salt solution (BSS) was used as control. Slit-lamp examination and histopathological examination was used to evaluated capsule opacifica-tion. Intraocular pressure (IOP) , density and morphology of corneal endothelia cells, the amplitude and latency of b wave of ERG were measured.Results: The inflammatory response was mild both in TAH treated and the control group. PCO formation occurred in the control group 2 weeks postoperatively, but the posterior capsule was clear in TAH treated eyes. 4 weeks and 8 weeks after operation, PCO occurred both in TAH treated and control eyes. However, it was milder in the TAH treated eyes. IOP remained at the normal level in all eyes. There was no difference in the density of corneal endothelial cells, and the amplitude and latency of b wave of ERG between TAH treated and control eyes. Histopathological study revealed that lens epithelial proliferation occurred 4 weeks and the Soemmerings ring developed 8 weeks postoperatively in the control eyes. However, only mild vacuolization and pkynotic changes of lens epithelial cells were found in TAH treated eyes. Transmmis-sion electronic microscopy demonstrated that there were mild cytoplasm vacuolization and mitochondria swelling of lens epithelial cells in TAH treated eyes. Conclusion: The results suggest that liposome encapsulated TAH can inhibit metaplasia and proliferation of lens epithelial cells in the rabbit eye without obvious toxicity to the eye tissue. It may be used as a potential agent to prevent the development of PCO. Eye Science 1999 ;15;55-60.

关 键 词：囊状不透明体 抑制作用 生物碱 荚膜 哈马灵 脂质体 晶状体上皮细胞 

分 类 号：R776[医药卫生—眼科]