Electrophoretic Determination of Aqueous and Serum Neuron-specific Enolase in the Diagnosis of Retinoblastoma  

作　　者：Zhongyao Wu, Huasheng Yang, Suhua Pan, Zhicong ChenZhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences , Guangzhou 510060 , China 

出　　处：《眼科学报》1997年第1期12-16,共5页Eye Science

摘　　要：Purpose: Neuron-specific enolase (NSE) of containing γ-enolase is considered valuable in the diagnosis of tumours of neuroectodermal origin.Method : We used rapid electrophoretic method on cellulose acetate plate to determine the pattern of enolase isoenzymes in 21 aqueous humor and 23 serum specimens from retinoblastoma (Rb) and 21 aqueous and 25 serum specimens from 25 control cases to evaluate NSE in the diagnosis of Rb. The assay allowed assessment of all three major isoenzymes (aa,aγ and γγ),and NSE relative activity and its percentage in the total relative activity of the three enolase isoenzymes were assessed by means of fluorometer.Result: Aqueous from all patients with Rb contained aa,ar and rr isoenzymes and presented strong postitive, the positive rate of NSE being 100% and its relative activity accounting for 45 ± 9% of the total relative activity of the 3 enolase isoenzymes; No enolase was detectable in control aqueous with cataract, glaucoma and Coats's diseases (4 cases),but in twoPurpose: Neuron-specific enolase (NSE) of containing γ-enolase is considered valuable in the diagnosis of tumours of neuroectodermal origin. Method : We used rapid electrophoretic method on cellulose acetate plate to determine the pattern of enolase isoenzymes in 21 aqueous humor and 23 serum specimens from retinoblastoma (Rb) and 21 aqueous and 25 serum specimens from 25 control cases to evaluate NSE in the diagnosis of Rb. The assay allowed assessment of all three major isoenzymes (aa,aγ and γγ),and NSE relative activity and its percentage in the total relative activity of the three enolase isoenzymes were assessed by means of fluorometer. Result: Aqueous from all patients with Rb contained aa,ar and rr isoenzymes and presented strong postitive, the positive rate of NSE being 100% and its relative activity accounting for 45 ± 9% of the total relative activity of the 3 enolase isoenzymes; No enolase was detectable in control aqueous with cataract, glaucoma and Coats's diseases (4 cases),but in two patients with traumatic hyphema eta and faint ay and yy were presented in aqueous. The control serum contained only an aa band, while the sera form patients with Rb contained aa, ay and yy bands in 18 of 23 specimens, the positive rate being 78. 2% ,and only aa bands in the rest 5 specimens (21. 8%). In patients with Rb,the NSE relative activity and its percentage in the total relative activity of the 3 isoenzymes in serum (36 ± 9 %) were significantly lower lever than those in aqueous. Conclusion : The amounts of NSE increase significantly in both serum and aqueous from patients with Rb, and the immunoelectrophoretic assay for determination of enolase patterns is valuable in the diagnosis of Rb and differential diagnosis of Rb. In addition, the method is rapid, simple and requires only a little amount (<5μ L) of samples. Eye Science 1997; 13 : 12 - 16.

关 键 词：眼 免疫电泳 水状体 血清 成视网膜细胞瘤 神经元性烯醇酶 

分 类 号：R774.1[医药卫生—眼科] R739.7[医药卫生—临床医学]