胎脾LAK细胞杀伤不同分化程度的髓系白血病细胞系的比较  

Comparison of Fetal Killer(LAK)Activity in Different Differentiating Stages of Myeloid Leukemic Cell Lines

作　　者：胡国柱[1] 张进[1] 聂荣庆[1] 文珠[2] 江希照 

机构地区：[1]江西省中医药研究所中心实验室,南昌330077 [2]江西省医学科学研究所 [3]江西省婺源县人民医院

出　　处：《江西医学院学报》1998年第3期45-48,共4页Acta Academiae Medicinae Jiangxi

摘　　要：使用低浓度的重组人白介素-2（rhIL－2）诱导的胎脾LAK细胞杀伤髓系白血病细胞系HL－60和KG－Ia51Cr释放研究表明，在不同的胎脾LAK效应细胞和靶细胞比（E：T）条件下，对两种分化程度不同的白血病细胞杀伤能力也不同。E：T越高杀伤能力越强，HL－60P＜0．01，KG-IaP＜0．005。胎脾LAK细胞杀伤HL－60平均杀伤率为55．44％±11．23％，KG－Ia平均杀伤率为22．54％±8．22％，两者比较P＜0．005。在相同E：T时对HL－60和KG－Ia杀伤活性比较P＜0．002。结果提示，胎脾LAK细胞对分化程度好的白血病细胞HL－60杀伤能力显著高于分化差的原始白血病细胞KG－Ia，E：T越大杀伤活性越强。因而为LAK细胞治疗髓系白血病提供了参考。Study of fetal spleen LAK cells induced by low concentration rhIL-2 killing 51Cr-markedmyeloid leukemic cell lines,HL-60 and KG-Ia demnnstrated that capacity of LAK cells inkilling HL-60 and KG-Ia varied under different ratios between fetal spleen LAK cells andtarget cells (E: T). The higher the E: T ratio,the greater the killer capacity, with significant difference P<0. 01 in HL-60 and P<0. 005 in KG-Ia. The fetal spleen LAK capacitywas also different in leukemic cells with different differentiating degrees. The average killingrate of fetal spleen LAK was different in HL-60 (55. 44% ± 11. 23% )from that in KG-Ia (22.54% ±8.22% ),P<0. 005. while under the same ratio of E: T,P<0. 002. The results suggest that the killer activity of fetal spleen LAK is higher in well-differentiated myeloidleukemic cell HL-60 than that of low-differentiated myeloid leukemic cell KG-Ia. This studyprovides a reference for the treatment of myeloid leukemia with LAK cells.

关 键 词：粒细胞白血病 免疫学 淋巴因子激活 白细胞介素2 淋巴细胞性白血病 RHIL-2 ALL AML 

分 类 号：R733.7[医药卫生—肿瘤]